高压热杀菌灭活枯草杆菌芽孢的作用研究  

Studies on Inactivation of HPTS on Bacillus subtilis Spore

作　　者：申瑾 谭睿 郭家俊 陈翔 吴珊 包军鹏 章中[1] Shen Jin;Tan Rui;Guo Jiajun;Chen Xiang;Wu Shan;Bao Junpeng;Zhang Zhong(College of Agriculture,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《中国食品学报》2022年第6期142-152,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(3176100462)。

摘　　要：为探究高压热杀菌(HPTS)对枯草杆菌芽孢灭活的影响,采用200 MPa结合25,65,75℃;550 MPa结合25,65,75℃;保压时间:20 min处理枯草杆菌芽孢,通过平板技术法、紫外分光光度法、流式细胞术、红外光谱法分析HPTS处理前、后芽孢的存活浓度、紫外吸收泄露量、内膜通透性、芽孢成分。结果显示:HPTS处理后,芽孢的存活浓度显著降低(P<0.05),紫外吸收泄漏量增大,芽孢内膜受损,芽孢内膜通透性增强。傅里叶红外光谱结果显示:HPTS处理前、后枯草杆菌芽孢的差异主要表现在蛋白质、脂质、核酸、多糖4种成分及其结构发生变化,如蛋白质二级结构从有序向无序转变,蛋白质稳定性降低。HPTS可有效破坏芽孢内膜的水分子通透屏障,改变芽孢膜脂质、蛋白质、核酸、多糖结构,从而使芽孢的抗性降低,影响芽孢的代谢,使芽孢灭活。本研究结果为HPTS灭活枯草杆菌芽孢提供理论依据,促进HPTS方法在食品加工中的应用。In order to explore the mechanism of HPTS in inactivation of Bacillus subtilis spores.In this paper,HPTS(200 MPa combined with 25℃,65℃,75℃;550 MPa combined with 25℃,65℃,75℃;holding time:20 min)was used to treat Bacillus subtilis spores.The survival concentration,UV absorption leakage,inner membrane permeability and spore composition of HPTS before and after HPTS treatment were studied by plate technology,UV spectrophotometry,flow cytometry and infrared spectroscopy.The results showed that after treated with HPTS,the survival concentration of spores decreased significantly(P<0.05),the leakage of ultraviolet absorption increased,and the endospore membrane was damaged,resulting in the enhancement of the permeability of spore endomembrane.The results of Fourier transform infrared spectroscopy(FTIR)showed that the main differences of Bacillus subtilis spores before and after HPTS treatment were protein,lipid,nucleic acid and polysaccharide.The secondary structure of protein changed from order to disorder,and the stability of protein decreased.Therefore,HPTS can effectively destroy the permeability barrier of water molecules,lipid,protein,nucleic acid and polysaccharide structure of spore membrane,so as to reduce the resistance of spores,affect the metabolism of spores and inactivate the spores.It provides a theoretical basis for HPTS to inactivate Bacillus subtilis spores and promotes the successful application of HPTS in food processing.

关 键 词：高压热杀菌 傅里叶红外光谱 芽孢 机理 灭活 

分 类 号：TS201.3[轻工技术与工程—食品科学]