基于免疫层析图像可视化的黄曲霉毒素B_(1)快速定量方法  

Rapid Quantitative Method for the Determination of Aflatoxin B_(1) Based on CCD

作　　者：郑允允 毛佳琦 吕俊 王培 谢会君 付玉洁 Zheng Yunyun;Mao Jiaqi;LüJun;Wang Pei;Xie Huijun;Fu Yujie(Zhejiang University Zhongyuan Institute,Zhengzhou 450001)

机构地区：[1]浙江大学中原研究院,郑州450001

出　　处：《中国食品学报》2022年第6期259-266,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(50309011)。

摘　　要：黄曲霉毒素B_(1)是一种毒性最大的黄曲霉毒素,与人类肝癌的病因有关。以黄曲霉毒素B_(1)胶体金检测卡为载体,将黄曲霉毒素B_(1)检测信息通过胶体金检测技术进行可视化呈现。采用电荷耦合器件记录检测信息,利用颜色空间进行数学建模,并将可视化信息进行数字化提取。通过建立的模型途径将获取黄曲霉毒素B_(1)含量的信息进行识别与表达。结果表明:制备的黄曲霉毒素B_(1)胶体金检测卡T线和C线的颜色距离与AFB_(1)样品浓度呈线性相关(R^(2)=0.986)。图像信息显示,质量浓度为20μg/kg的平行样间的相对标准偏差小于2.7%的点占97%;质量浓度为10μg/kg时,相对标准偏差小于3.6%的点为90%;质量浓度为5μg/kg相对标准偏差小于4%的点达到96%以上。本研究方法获取的线性结果与人工试验所得的线性结果间最大误差为5.93%,与高效液相色谱试验结果对比,无显著性差异(P>0.05)。该方法能够同时对多个层析检测卡样本信息进行记录,实现一次检测试验可以完成多个样品多指标定量检测和结果输出,为黄曲霉毒素B_(1)的快速定量提供了一种有效途径。Aflatoxin B_(1)(AFB_(1))is a predominant toxin detected abundantly and has been implicated in the etiology of human hepatocellular carcinoma.In this study,a novel quantitative recognition strategy of Aflatoxin B_(1)was proposed.The reaction medium for AFB_(1)was built using colloidal gold immuno-chromatography and information of the results was obtained using the technology of charge coupled device(CCD).An expressing method called color space was established to express the information of AFB_(1).The path can be used to obtain accurate measurement of the AFB_(1).The results showed that the color distance of T line and C line(AFB_(1)test card)was linearly correlated with the concentration(R^(2)=0.986).The precision of the experiments was as follows:the relative standard deviation of the high concentration(20μg/kg)was<2.7%for more than 97%dots,and that of the other two samples was<3.6%for more than 90%dots.This showed that the degree of coincidence of information in pixels between parallel color images(the same sample)was high.The maximum error between the linear results obtained by machine learning methods and the linear results obtained by 30 groups of parallel experiments is 5.93%,additionally,the results had no significant difference between RqM-AFB_(1)and HPLC test(P>0.05).Thus,this method provides an effective way to improve fast quantitative and accurate identification of Aflatoxin B_(1).

关 键 词：黄曲霉毒素B_(1) 胶体金检测卡 图像信息识别 快速定量 

分 类 号：TS207.5[轻工技术与工程—食品科学]