金针菇高表达基因的密码子偏好性及特征分析  

Codon preference and characteristic analysis of the genes highly expressed in Flammulina filiformis

作　　者：陈奕钊 李晓宇 吴家来 胡佳 刘媛媛 叶新怡 李慧[3] 陶永新[1,2] CHEN Yizhao;LI Xiaoyu;WU Jialai;HU Jia;LIU Yuanyuan;YE Xinyi;LI Hui;TAO Yongxin(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Mycological Research Center,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,Hebei,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学生命科学学院菌物研究中心,福建福州350002 [3]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《菌物学报》2022年第6期939-951,共13页Mycosystema

基　　金：国家自然科学基金(31902088);福建省自然科学基金(2019J01380);福建农林大学杰出青年科研人才计划项目(xjq201919);福建农林大学科技创新专项基金(CXZX2017184);福建省种业创新与产业化工程项目(zycxny2021012);河北省重点研发计划项目(19226368D,21326315D)。

摘　　要：为探究金针菇的密码子偏好性,挖掘高表达基因的特征信息,以金针菇基因组及转录组数据为材料,分析金针菇的密码子偏好性及其影响因素,并对发育阶段高表达基因进行功能注释和顺式元件分析。分析表明,金针菇高表达基因表现出较强的密码子偏好性,且其偏好密码子多以胞嘧啶(C)结尾,此外在高表达基因中存在6种氨基酸的最优密码子较为保守。在进化过程中,金针菇高表达基因密码子偏好性受到自然选择压力的影响较大。功能注释分类表明,高表达基因多为核糖体通路相关的基因,与蛋白质翻译和生物合成相关。顺式元件分析表明,高表达基因启动子区域大多存在MeJA响应元件、ABA响应元件、光响应元件及MYB转录因子结合元件。研究结果可为提高金针菇异源表达效率和挖掘强启动子提供理论基础和思路。Codon preference and characteristics of the gene highly expressed during developments of Flammulina filiformis were explored.Genome and transcriptome data of F.filiformis were used to analyze the codon preference and its influencing factors,and the functional annotation and cis-element analysis of high expression genes were carried out.The results showed that high expression genes of F.filiformis showed strong codon preference,and most preferred codons ended with cytosine(C).The optimal codons of six amino acids in the high expression genes were conservative.In the evolutionary process,codon preference of high expression genes of F.filiformis was greatly affected by natural selection pressure.Functional annotation classification shows that high expression genes are mostly ribosomal pathway related genes in relation to protein translation and biosynthesis.The cis-element analysis showed that MeJA response element,ABA response element,light response element and MYB transcription factor binding element were mostly presented in the promoter region of high expression genes.The results provide theoretical basis and ideas for improving heterologous expression efficiency and strong promoter mining of F.filiformis.

关 键 词：金针菇 密码子偏好性 高表达基因 

分 类 号：S646.15[农业科学—蔬菜学]