皱环球盖菇不同极性萃取物抗氧化活性比较及成分分析  

Comparison of antioxidant activities and constituent analyses of different polar extracts from Stropharia rugosoannulata

作　　者：吕国英[1] 宋婷婷[1] 贺伟强[2] 张洋洋 蔡为明[1] 张作法[1] LV Guoying;SONG Tingting;HE Weiqiang;ZHANG Yangyang;CAI Weiming;ZHANG Zuofa(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China;Jiaxing Vocational and Technical College,Jiaxing 314001,Zhejiang,China)

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021 [2]嘉兴职业技术学院,浙江嘉兴314001

出　　处：《菌物学报》2022年第6期999-1007,共9页Mycosystema

基　　金：浙江省农业新品种选育重大科技专项(2021C02073);国家现代农业技术体系(CARS-20)。

摘　　要：为考察皱环球盖菇子实体的抗氧化活性,利用溶剂萃取法得到皱环球盖菇子实体二氯甲烷、乙酸乙酯、正丁醇和水4种不同极性萃取物,选用二苯基三硝基苯肼自由基(DPPH)法、羟基自由基法和还原力法对4种萃取物进行体外抗氧化活性分析。结果表明,乙酸乙酯萃取物显示了最强的抗氧化活性;通过超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-Triple-TOF/MS)分析了皱环球盖菇乙酸乙酯萃取物中化学组成,从中鉴定出9种化合物,多数为核苷类物质,具有很好的抗氧化活性。研究结果为皱环球盖菇的开发提供了理论依据和技术支持。The chemical constituents of fruiting body of Stropharia rugosoannulata was extracted by dichloromethane,ethyl acetate(EtOAc),n-butanol and water.Diphenyl trinitrophenyl hydrazine free radical(DPPH),hydroxyl free radical and reducing power methods were used to analyze the antioxidant activities of the four extracts in vitro.The EtOAc extract showed the strongest antioxidant activity as compared with other extracts.Chemical constituents of the EtOAc extract were analyzed using ultra-high performance liquid chromatography tandem triple TOF-MS(UPLC-Triple-TOF/MS).Nine compounds were identified as nucleoside analogues,which displayed good antioxidant activities.The results provide a reference for further development of S.rugosoannulata.

关 键 词：皱环球盖菇 不同极性提取物 抗氧化活性 核苷类物质 超高效液相色谱串联三重四级杆飞行时间质谱分析 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]