UPLC-MS/MS法测烘焙咖啡和速溶咖啡中丙烯酰胺  

Determination of Arcylamide in Roast Coffee and Instant Coffee by UPLC-MS/MS

作　　者：田金凤[1] 张喆 孙卓然[2] 尚远宏[1] TIAN Jinfeng;ZHANG Zhe;SUN Zhuoran;SHANG Yuanhong(College of Medicine,Panzhihua University,Panzhihua 617000;Hohhot Center for Disease Control and Prevention,Hohhot 010070)

机构地区：[1]攀枝花学院医学院,攀枝花617000 [2]呼和浩特市疾病预防控制中心,呼和浩特010070

出　　处：《食品工业》2022年第4期322-325,共4页The Food Industry

基　　金：四川省科技厅项目(2021YFN0101);攀枝花市科学技术局指导性科技计划项目(2020ZD-S-32,2020ZD-N-2)。

摘　　要：采用超高效液相色谱串联三重四极杆质谱法测定烘焙咖啡和速溶咖啡中丙烯酰胺。样品2.0 g经正己烷去脂和乙腈提取,MCX固相萃取柱净化,采用Waters BEH-C18色谱柱分离,以0.1%的甲酸水溶液和乙腈溶液为流动相进行梯度洗脱;质谱采用电喷雾离子源和多反应监测模式。结果显示,当丙烯酰胺的质量浓度为5.0~250.0μg/L时,线性关系良好,相关系数(r)>0.999,定量限为1.0μg/kg,空白样品在3个添加水平(1,5和10μg/kg)下的回收率为81.2%~89.3%,S_(RSD)为5.1%~6.3%(n=6)。该方法灵敏度及准确度良好,能满足咖啡中丙烯酰胺含量检测的要求。This study investigated to determine of arcylamide in roast coffee and instant coffee by ultra-performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS).After 2.0 g samples were degreased with n-hexane,arcylamide was extracted with acetonitrile,and cleaned up on a MCX cartridge.The target analytes were separated on a Waters BEH-C18 column with gradient elution using a mobile phase made up of 0.1%formic acid solution and acetonitrile.Electrospray ionization(ESI)and multiple reaction monitoring(MRM)mode were adopted in MS analysis.The results showed that concentration of arcylamides showed good linearity in the range of 5.0-250.0μg/L(r>0.999).The limits of detection were 1.0μg/kg.The spiked recovery rates were in the range of 81.2%-89.3%at 3 spiked levels(1,5 and 10μg/kg)for blank sample,and the relative standard deviation(S_(RSD))was 5.1%-6.3%(n=6).The method was sensitive and accurate.It could meet the requirements for the determination of arcylamide in coffee.

关 键 词：超高效液相色谱串联三重四极杆质谱 丙烯酰胺 烘焙咖啡 速溶咖啡 

分 类 号：O657.63[理学—分析化学] TS273[理学—化学]