黄曲霉毒素B1光纤检测系统设计  

Design of optical fiber detection system for aflatoxin B1

作　　者：胡良 吴才章[1] 赵志科 徐坤 Hu Liang;Wu Caizhang;Zhao Zhike;Xu Kun(College of Electrical Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学电气工程学院,郑州450001

出　　处：《电子测量技术》2022年第4期39-44,共6页Electronic Measurement Technology

基　　金：河南省科技攻关项目(182102110461);河南工业大学创新基金支持计划专项(2020ZKCJ31)资助。

摘　　要：为了解决传统化学发光酶免疫分析方法中大尺寸光电检测装置探测头与反应孔的对准难题,采用多模光纤结合光纤型光子计数探头对目标信号进行探测,设计一种基于光电检测技术的黄曲霉毒素B1光纤检测系统。通过ZEMAX仿真与测试结果显示,在反应孔范围内,与反应孔中心位置检测相比光纤光子收集比达到95.83%以上,检测相对标准偏差RSD≤0.21%,光纤探测口与反应孔对准要求大大降低。试验结果表明,该光纤检测系统的检出限为0.63μg/L,标准曲线相关系数为0.9977,RSD为1.03%~2.38%,样品加标回收率为92.62%~96.03%,具有更高线性度、精密度、重现性等。In order to solve the alignment problem between the detection head of the large-size photoelectric detection device and the reaction hole,a small,in the traditional chemiluminescence enzyme immunoassay method.A multi-mode optical fiber combined with the optical fiber-type photon counting probe is used to detect the target signal,and an aflatoxin B1optical fiber detection system based on photoelectric detection technology is designed.The ZEMAX simulation and test results show that the optical fiber photon collection ratio reaches more than 95.83%and RSD≤0.21%in the reaction hole range compared with the reaction hole center position detection,and the alignment requirements of the optical fiber detection port and the reaction hole are greatly reduced.The experimental results showed that the detection limit of the fiber optic detection system was 0.63μg/L.The correlation coefficient of the standard curve was 0.9977.The RSD was 1.03%~2.38%,and the sample spiked recovery was 92.62%~96.03%with higher linearity,precision,and reproducibility.

关 键 词：黄曲霉毒素B1 光电检测 多模光纤 ZEMAX仿真 

分 类 号：TN247[电子电信—物理电子学] S24[农业科学—农业电气化与自动化]