阿尔茨海默病血清代谢物的核磁共振氢谱技术分析  

Analysis of serum metabolites in Alzheimer's disease by 1H nuclear magnetic resonance

作　　者：郑锦仁 迪娜·库勒拜 王彩丽[2] 马晓洁[2] 买买提热厦提·吐尔逊[2] 张文惠[2] Zheng Jinren;Dina Kulebai;Wang Caili;Ma Xiaojie;Maimaitirexiati Tuerxun;Zhang Wenhui(Clinical Laboratory of Urumqi Hospital of TCM,Urumqi,Xinjiang 830000,China;Second Department of Geriatrics,Urumqi Fourth People's Hospital,Urumqi,Xinjiang 830002,China)

机构地区：[1]乌鲁木齐市中医医院检验科,新疆乌鲁木齐830000 [2]乌鲁木齐市第四人民医院老年二科,新疆乌鲁木齐830002

出　　处：《现代临床医学》2022年第4期253-256,共4页Journal of Modern Clinical Medicine

基　　金：2014年乌鲁木齐市卫生局科技计划项目(201404)。

摘　　要：目的:通过核磁共振氢谱技术(1H-NMR)分析阿尔茨海默病(AD)患者的血清代谢物,以期寻找AD稳定、可靠的早期诊断标志物。方法:将2014年4月至2016年4月就诊于乌鲁木齐市第四人民医院的AD患者50例作为AD组,纳入同期健康体检者50例作为对照组。应用1H-NMR检测其血清代谢物,采用正交偏最小二乘判别分析法分析代谢物的分布特征,并在构建代谢模型后,对两组的血清差异代谢物进行代谢通路富集分析。结果:与健康体检者比较,AD患者血清中亮氨酸、瓜氨酸、脯氨酸、异亮氨酸、蛋氨酸、缬氨酸、丙氨酸、β-葡萄糖、乳酸及极低密度脂蛋白等代谢产物的水平差异有统计学意义(P<0.05)。结论:AD患者血清氨基酸、糖及脂类代谢紊乱,尤以必需氨基酸较为明显,提示AD的发生与能量代谢失衡相关。Objective:To analyze the serum metabolites of patients with Alzheimer's disease(AD)by 1H nuclear magnetic resonance(1H-NMR)in order to find stable and reliable early diagnostic markers for AD.Methods:Fifty AD patients hospitalized in Urumqi Fourth People's Hospital from April 2014 to April 2016 were included in the AD group,and 50 healthy subjects during the same period were included in the control group..Their serum metabolites were detected by 1H-NMR.The distribution characteristics of metabolites were analyzed by orthogonal partial least-squares discriminant analysis.After constructing the metabolic model,the serum differential metabolites in the two groups received enrichment analysis of metabolic pathways.Results:The serum levels of metabolites such as leucine,citrulline,proline,isoleucine,methionine,valine,alanine,β-glucose,lactic acid,and very low-density lipoprotein in AD patients were significantly different compared with those in healthy subjects(P<0.05).Conclusion:The metabolism of amino acids,sugars and lipids in serum of AD patients is disordered,especially essential amino acids,which suggests that the occurrence of AD is related to the imbalance of energy metabolism.

关 键 词：阿尔茨海默病 代谢组学 核磁共振氢谱 

分 类 号：R749.16[医药卫生—神经病学与精神病学]