胰腺导管腺癌中PDIA6和PD-L1的表达及其临床病理学意义  

Expression of PDIA6 and PD-L1 in pancreatic ductal adenocarcinoma and their clinicopathological significance

作　　者：金汝佳 王丰[1] 李晨飞[1] 马怡晖[1] JIN Ru-jia;WANG Feng;LI Chen-fei;MA Yi-hui(Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)

机构地区：[1]郑州大学第一附属医院病理科,郑州450052

出　　处：《临床与实验病理学杂志》2022年第6期663-668,共6页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)中PDIA6和PD-L1的表达及与临床病理学特征和预后的相关性。方法收集2013年1月~2019年12月郑州大学第一附属医院确诊的79例PDAC,采用免疫组化SP法检测PDIA6和PD-L1蛋白在PDAC中的表达,分析两者表达与临床病理特征及预后的关系。结果79例PDAC中PDIA6和PD-L1的阳性率分别为57.0%(45/79)和7.6%(6/79),且PDIA6和PD-L1在PDAC中的表达均显著高于癌旁正常组织(P<0.05)。PDIA6表达与肿瘤出芽有关(P=0.035),与患者性别、年龄、肿瘤直径、肿瘤部位、分化程度、TNM分期、间质肿瘤浸润淋巴细胞、脉管内癌栓、神经侵犯、淋巴结转移等均无关(P>0.05);PD-L1表达与PDAC临床病理特征均无关(P>0.05)。PDAC预后的单因素生存分析结果显示,PDIA6(P=0.009)和PD-L1(P=0.040)表达与PDAC患者预后不良相关。此外,肿瘤出芽(P=0.016)、淋巴结转移(P<0.001)以及神经侵犯(P=0.012)均与总生存期较短有关。Cox多因素生存分析显示,PDIA6表达、PD-L1表达、肿瘤出芽和淋巴结转移均是影响PDAC患者总生存期的独立危险因素。结论PDIA6、PD-L1表达与PDAC患者较短的总生存期有关,可作为PDAC患者预后不良的指标。Purpose To investigate the expression of PDIA6 and PD-L1 in pancreatic ductal adenocarcinoma(PDAC)and their correlation with clinicopathologic features and prognosis.Methods A total of 79 patients with PDAC by pathologically diagnosed after surgical resection in the First Affiliated Hospital of Zhengzhou University from January 2013 to December 2019.The clinical pathological data of patients were collected and the prognosis of patients were followed up by telephone.The expression of PDIA6 and PD-L1 protein in PDAC was detected by SP immunohistochemical methods,and their relationship with clinicopathological characteristics and prognosis of patients were analyzed.Results The positive rates of PDIA6 and PD-L1 in 79 cases of PDAC were 57.0%(45/79)and 7.6%(6/79),respectively and the expression of PDIA6 and PD-L1 in PDAC was significantly higher than that in adjacent normal pancreatic duct(P<0.05).PDIA6 expression was associated with tumor budding(P=0.035),but had no significant correlation with gender,age,tumor size,location,differentiation degree,TNM stage,interstitial tumor infiltrating lymphocytes,tumor thrombus,nerve invasion,and lymph node metastasis(P>0.05).There was no significant correlation between PD-L1 expression and clinical pathological features of patients(P>0.05).Univariate analysis of PDAC prognostic factors showed that PDIA6(P=0.009)and PD-L1(P=0.040)expression was associated with poor prognosis in PDAC patients.In addition,tumor budding(P=0.016),lymph node metastasis(P<0.001),and nerve invasion(P=0.012)were all associated with shorter overall survival of patients.Cox multivariate survival analysis showed that patients with PDIA6 expression,PD-L1 expression,tumor budding and lymph node metastasis were all independent risk factors affecting the overall survival of PDAC patients.Conclusion The positive expression of PDIA6 and PD-L1 is related to the shorter overall survival of patients with PDAC,which can be used as predictors of poor prognosis of patients with PDAC.

关 键 词：胰腺肿瘤 导管腺癌 PDIA6 PD-L1 临床病理特征 预后 

分 类 号：R735.9[医药卫生—肿瘤]