新型染料木素衍生物对肺癌细胞活性影响的研究  

On the Effect of New Genistein Derivatives on the Activity of Lung Cancer Cells

作　　者：赵芳 李红侠 刘宇 鲍道源 ZHAO Fang;LI Hongxia;LIU Yu;BAO Daoyuan(School of Biological and Food Engineering,Suzhou University,Suzhou 234000,China)

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《宿州学院学报》2022年第6期26-30,共5页Journal of Suzhou University

基　　金：安徽省高校自然科学基金项目(KJ2021A1109);宿州学院科研平台项目(2020ykf24);宿州学院大学生科研项目(KYLXYBXM21-057;KYLXYBXM21-053)。

摘　　要：研究新型染料木素衍生物对肺癌细胞活性的影响,以染料木素为原料,通过与哌嗪反应合成目标产物(GeD)。经^(1)H NMR、IR等对目标物确证。MTT比色法检测药物对人非小细胞肺癌细胞的抗增殖活性,流式细胞仪检测药物对人非小细胞肺癌细胞凋亡率。结果GeD对人非小细胞肺癌细胞具有较好的抗增殖活性,48 h的IC_(50)值20.32μM,细胞凋亡率呈时间和剂量依赖性。The effect of new genistein derivatives on the activity of lung cancer cells is studied.Using genistein as raw material,the target product(GeD)is synthesized by reacting with piperazine.The target was confirmed by ^(1)H NMR and IR.MTT colorimetric method was used to detect the anti-proliferative activity of drugs on human non-small cell lung cancer cells,and flow cytometry was used to detect the apoptosis rate of drugs on human non-small cell lung cancer cells.Results show that GeD has good anti-proliferative activity against human non-small cell lung cancer cells,with an IC_(50) value of 20.32μM at 48 h,and the apoptosis rate is time and dose-dependent.

关 键 词：染料木素 衍生物 抗癌活性 

分 类 号：R574[医药卫生—消化系统]