高效液相色谱法检测药品糖浆剂中4种人工合成甜味剂  

Determination of four kinds of illegally-added artificial sweeteners in syrups by high performance liquid chromatography

作　　者：钟菲菲 雷德卿 周金沙 刘波 王子倩 ZHONG Fei-fei;LEI De-qing;ZHOU Jin-sha;LIU Bo;WANG Zi-qian(Changsha Institute for Food and Drug Control,Changsha,Hunan 410008,China)

机构地区：[1]长沙市食品药品检验所,湖南长沙410008

出　　处：《食品与机械》2022年第7期75-79,共5页Food and Machinery

基　　金：湖南省自然科学基金项目(编号:2021JJ80076)。

摘　　要：目的:建立高效液相色谱法同时测定糖浆剂药品中安赛蜜、糖精钠、阿斯巴甜、爱德万甜4种人工合成甜味剂。方法:以乙腈为流动相A,以0.02 mol/L磷酸二氢铵(用三乙胺调节pH值至5.5)为流动相B,梯度洗脱,流速为1.0 mL/min,柱温为35℃,检测波长为214 nm,进样量为10μL,色谱柱为Shiseido AQ-C_(18)(4.6 mm×250 mm,5μm);用建立的方法测定21家生产企业共45批次小儿止咳糖浆样品。结果:4种甜味剂在2~100 mg/L范围内线性关系良好,安赛蜜、糖精钠、阿斯巴甜、爱德万甜平均回收率分别为98.65%,104.09%,100.96%,102.30%,RSD均小于2%(n=9);实际样品检测结果显示3批次样品被检出爱德万甜,2批次样品被检出阿斯巴甜和爱德万甜。结论:所建立的方法专属性强,简单可靠,可用于糖浆剂药品非法添加人工合成甜味剂的监测。Objective:A high performance liquid chromatography method was established for determination of four artificial sweeteners in syrups,i.e.,acesulfame,saccharin sodium,aspartame and advantame.Methods:Acetonitrile was used as mobile phase A,and 0.02 mol/L ammonium dihydrogen phosphate(pH value adjusted to 5.5 by triethylamine)was used as mobile phase B.The gradient elution was conducted,with flow rate at 1.0 mL/min,and the column temperature was at 35℃;the detection wavelength was 214 nm,and sample size was 10μL was loaded on the was Shiseido AQ-C_(18)column(4.6 mm×250 mm,5μm)A total of 45 batches of children’s cough syrup samples from 21 manufacturers were determined by the established method.Results:The linear calibration curve of the four kinds of artificial sweeteners were obtained over the range of 2~100 mg/L.The average recoveries of acesulfame,saccharin sodium,aspartame and advantame were 98.65%,104.09%,100.96%and 102.30%respectively,with RSDs less than 2%(n=9).The actual sample test results showed that 3 batches of samples were found to be advename,and 2 batches of samples were found to be aspartame and advename.Conclusion:The established method is specific,simple,and reliable,and can be used to monitor the illegal addition of artificial sweeteners in syrups.

关 键 词：高效液相色谱法 糖浆剂 人工合成甜味剂 安赛蜜 糖精钠 阿斯巴甜 爱德万甜 

分 类 号：O657.72[理学—分析化学] TQ460.72[理学—化学]