基于孢粉素微胶囊的共包埋、保护及递送益生菌和乳糖酶的体系  

Sporopollenin Microcapsule-based System for Co-encapsulation,Protection and Delivery of Probiotics andβ-Galactosidase

作　　者：邓紫玙 丁一 侯欣尧 李斌[1] 梁宏闪 DENG Ziyu;DING Yi;HOU Xinyao;LI Bin;LIANG Hongshan(Key Laboratory of Environment Correlative Dietology,Ministry of Education,College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科技学院,环境食品学教育部重点实验室,湖北武汉430070

出　　处：《食品科学》2022年第14期183-192,共10页Food Science

基　　金：湖北省本科生科技创新专项(201510318255);国家自然科学基金青年科学基金项目(31801586)。

摘　　要：研究旨在开发基于天然植物微胶囊的核壳结构载体,作为益生菌和乳糖酶的递送及保护体系。该核壳结构的孢粉素外壁胶囊(sporopollenin exine capsules,SECs)为核,海藻酸钙(Ca-alginate,Ca-Alg)/羧甲基茯苓多糖(carboxymethylpachymaran,CMP)凝胶为壳,对益生菌的负载量高达9.63×10^(9) CFU/g,乳糖酶活力保留率高达80.72%。在Ca-Alg/CMP壳中,CMP引入可以改变凝胶壳层对水的结合能力,从而改变其溶胀行为和微观结构,借此影响体系中益生菌和乳糖酶的释放行为。结果表明,引入CMP可使Ca-Alg/CMP凝胶包裹的负载益生菌及乳糖酶的SECs在胃肠道中表现出更加优异的稳定性:在模拟消化600 min后,活菌数超过10^(7) CFU/mL,酶活力保留率约为62%。相比于纯菌液和纯酶液,该体系可减少冻干和贮存处理后菌数和酶活力损失率(P<0.05)。最后,利用甲基纤维素对核壳结构进行二次包埋,可提高体系中益生菌和乳糖酶的热稳定性(P<0.05)。This study aimed to develop a core-shell carrier based on natural plant microcapsules for use as a delivery system for Lactobacillus plantarum andβ-galactosidase(β-Gal).Sporopollenin exine capsules(SECs)were used as the core,and calcium-alginate(Ca-Alg)/carboxymethylpachymaran(CMP)as the shell.The probiotics loading capacity and the residual activity ofβ-Gal were 9.63×10^(9) CFU/g and 80.72%,respectively.The introduction of CMP could affect the swelling behavior and microstructure of the shell and,consequently,the release behavior of probiotics and enzymes by changing its water-binding capacity.The introduction of CMP improved the gastrointestinal stability of Ca-Alg/CMP coated SECs loaded with L.plantarum andβ-Gal.After 600 min of culture under simulated gastrointestinal conditions,the number of viable cells exceeded 10^(7) CFU/mL,and the residual activity ofβ-Gal was around 62%.Compared with the control group(pure L.plantarum andβ-Gal),the system could significantly reduce the loss rates of the probiotic cell count andβ-Gal activity after lyophilization and during storage(P<0.05).Finally,using methylcellulose(MC)to coat the core-shell structure could significantly improve the thermal stability of L.plantarum andβ-Gal(P<0.05).

关 键 词：孢粉素外壁胶囊 核壳结构 羧甲基茯苓多糖 益生菌 乳糖酶 稳定性 

分 类 号：TS201.4[轻工技术与工程—食品科学]