发菜藻粉对小鼠肠道菌群及免疫调节的影响  

Effect of Nostoc flagelliforme Powder on Intestinal Flora and Immune Regulation in Mice

作　　者：陈旎 武毅凯 李艳茹 王帅 韩成凤 李健[1] 王晓丽 张乐乐 谭之磊[1] 贾士儒[1] 韩培培[1] CHEN Ni;WU Yikai;LI Yanru;WANG Shuai;HAN Chengfeng;LI Jian;WANG Xiaoli;ZHANG Lele;TAN Zhilei;JIA Shiru;HAN Peipei(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《食品科学》2022年第13期55-63,共9页Food Science

基　　金：国家自然科学基金面上项目(31671842)。

摘　　要：目的:探究发菜藻粉对小鼠肠道菌群及免疫调节的影响。方法:对野生发菜藻粉和液体培养发菜藻粉进行化学组成的测定,用不同剂量的发菜藻粉(200、400 mg/kg mb)对小鼠进行灌胃,检测小鼠的体质量、免疫器官指数、免疫因子中白细胞介素1β(interleukin-1β,IL-1β)质量浓度、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)质量浓度、干扰素γ(interferon-γ,IFN-γ)质量浓度和分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)质量浓度以及免疫器官中免疫因子的表达量、肠道内容物的水分质量分数、pH值和短链脂肪酸含量,并评价发菜藻粉对小鼠肠道菌群及机体免疫的影响。结果:液体培养发菜藻粉营养价值更高,液体培养发菜藻粉高剂量组与对照组相比,免疫调节效果更为明显,提高了小鼠的器官指数,增加血清中IFN-γ、TNF-α及肠液中SIgA的质量浓度;明显上调脾脏内IFN-γ、TNF-α及血清型免疫球蛋白A(immunoglobulin A,IgA)的mRNA表达,促进相关免疫因子的合成;降低肠液的pH值,提高盲肠内容物中短链脂肪酸含量;提高小鼠肠道微生物的物种丰度和群落多样性,灌胃发菜藻粉使结肠与盲肠中拟杆菌门相对丰度降低,结肠中厚壁菌门相对丰度增加,另外,肠道中疣微菌门的相对丰度明显增加;与免疫相关的益生菌Akkermansia、Parabacteroides、Alistipes和Ruminiclostridium的相对丰度明显升高。结论:液体培养发菜藻粉对小鼠肠道菌群具有调节作用以及能够提高机体免疫能力。This study investigated the effect of Nostoc flagelliforme powder on the intestinal flora and immune regulation of mice.The chemical composition of wild and liquid cultured N.flagelliforme powder was determined.Mice were gavaged with different doses of N.flagelliforme powder(200 and 400 mg/kg mb).Afterwards,body mass,immune organ indices,the serum concentrations of interleukin 1β(IL-1β),tumor necrosis factorα(TNF-α),interferonγ(IFN-γ)and the level of intestinal secretory immunoglobulin A(SIgA),cytokine expression in immune organs,and the water content,pH,and short-chain fatty acids in intestinal contents were measured and the effect of Nostoc flagelliforme powder on the intestinal microecology of mice was evaluated.The results showed that the nutritional value of liquid cultured N.flagelliforme powder was higher than that of wild N.flagelliforme powder.Compared to the control group,high-dose liquid cultured N.flagelliforme powder increased immune organ indexes,serum IFN-γand TNF-αlevels and intestinal SIgA levels,up-regulated the mRNA expression of IFN-γ,TNF-αand immunoglobulin A(IgA)in the spleen promoting the synthesis of relevant immune factors,reduced the pH of intestinal fluid,and increased the content of short-chain fatty acids in cecum contents.The intervention of N.flagelliforme powder improved the species richness and diversity of the intestinal microflora in mice,decreased the relative abundance of Bacteroidetes in the colon and cecum,and increased the relative abundance of Firmicutes in the colon,and the relative abundance of Verrucomicrobia and the immune-related probiotics Akkermansia,Parabacteroides,Alistipes and Ruminiclostridium.Conclusion:Liquid cultured N.flagelliforme powder can regulate the intestinal flora and improve immune function in mice.

关 键 词：发菜 肠道菌群 免疫因子 

分 类 号：TS201.4[轻工技术与工程—食品科学]