Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats:In vivo and in silico studies  

作　　者：Ahmed M.Fatani Othman A.S.Baothman Lobna S Shash Huda A.Abuaraki Mustafa A Zeyadi Salman B.Hosawi Hisham N.Altayb Mohamed K.Abo-Golayel 

机构地区：[1]Biochemistry Department,Faculty of Science,King Abdulaziz University,Jeddah,Saudi Arabia [2]Microbial Toxicology&Natural Products Center,Faculty of Science,King Abdulaziz University,Jeddah,Saudi Arabia [3]Pathology Department,Faculty of Medicine,Ain Shams University,Cairo,Egypt [4]Laboratory Animal Unit,King Fahad Medical Research Centre,King Abdulaziz University,Jeddah,Saudi Arabia [5]Ain Shams University Research Institute,Ain Shams University Hospitals,Ain Shams University,Cairo,Egypt

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第8期357-366,共10页亚太热带生物医学杂志（英文版）

基　　金：King Abdulaziz City for Science and Technology(KACST)for its financial support under grant number(1-18-03-009-0057).

摘　　要：Objective:To investigate the prophylactic efficacy of date palm fruit extract against doxorubicin-induced hepatotoxicity in Wistar albino rats.Methods:The rats were equally and randomly assigned to 6 groups:group 1(untreated control),group 2 and 3 given daily oral administration of prophylactic aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight,respectively,and group 4,5 and 6 intraperitoneally injected with doxorubicin at 15 mg/kg on day 30.Rats in group 5 and 6 received daily oral administration of aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight,respectively,for 30 d.The phytochemicals identified by GC-MS analysis were analyzed using in silico study.Antioxidant enzymes,liver enzymatic,biochemical parameters and histopathological analysis were determined to evaluate hepatoprotective activity of date extract.Results:Aqueous extract of date palm fruit significantly mitigated doxorubicin-induced changes in activities of liver enzymes,reduced reactive oxygen species levels,and suppressed lipid peroxidation and DNA damage.Moreover,aqueous extract of date palm fruit reduced doxorubicin-induced hepatic lesions.Molecular docking studies showed that most compounds of aqueous extract of date palm fruit identified via GC-MS had good interaction with proteins of human pregnane X receptor,oxygenase-1,and CYP2C9.Conclusions:The aqueous extract of date palm fruit mitigates doxorubicin-mediated DNA damage and hepatotoxicity,and restores normal liver function and may be a promising agent against the deleterious effects of doxorubicin.

关 键 词：Date palm Aqueous extract DOXORUBICIN HEPATOTOXICITY Rat Liver enzymes Reactive oxygen species Lipid peroxidation DNA damage GC-MS Molecular docking 

分 类 号：R285.5[医药卫生—中药学]