N^(6)-methyladenosine demethylase ALKBH5:a novel regulator of proliferation and differentiation of chicken preadipocytes  

作　　者：Qi Zhang Bohan Cheng Haixu Jiang Huili Zhang Hui Li 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction,Education Department of Heilongjiang Province,Harbin 150030,China [2]Key Laboratory of Chicken Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Harbin 150030,China [3]College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第1期55-63,共9页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation(No.31902142);the China Agriculture Research System of MOF and MARA(No.CARS-41).

摘　　要：Previous studies have reported that the N^(6)-methyladenosine demethylase ALKBH5 can regulate adipogenesis in humans.However,its function in birds remains unclear.In this study we aimed to explore the expression and function of the ALKBH5 gene in chicken adipose tissue.The results showed that ALKBH5 is widely expressed in various chicken tissues,and the expression of ALKBH5 is relatively higher in abdominal adipose tissue.In addition,the expression of ALKBH5 in abdominal adipose tissue of lean broilers is higher than that in fat broilers at 2 and 3 weeks of age.Moreover,the proliferation and differentiation of preadipocytes are associated with reduced and increased expression of ALKBH5,respectively.We also found that ALKBH5 knockout promotes preadipocyte proliferation,as evidenced by an increase in cell viability,DNA replication activity,G_(1)-S cell cycle progression,and the expressions of Ki67 and PCNA.Furthermore,after knockout of ALKBH5,the lipid droplet accumulation and the expression of PPAR_(γ),A-FABP,and FAS are reduced significantly.Thus,our results indicated that ALKBH5 is a novel regulator of proliferation and differentiation of chicken preadipocytes.

关 键 词：ALKBH5 CHICKEN PREADIPOCYTE proliferation DIFFERENTIATION 

分 类 号：Q952[生物学—动物学]