Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy  

作　　者：Xin Zhang Juan Chen Jiwen Hu Anna du Rietz Xiongyu Wu Ruilong Zhang Zhongping Zhang Kajsa Uvdal Zhangjun Hu 

机构地区：[1]Department of Physics,Chemistry,and Biology(IFM),Linköping University,Linköping SE 58183,Sweden [2]School of Chemistry and Chemical Engineering,Anhui University,Hefei 230601,China

出　　处：《Nano Research》2022年第7期6515-6521,共7页纳米研究（英文版）

基　　金：supported by STINT Joint China-Sweden Mobility Project(No.CH2017-7243);the Swedish Research Council(VR)(Nos.2019-02409 and 2020-05437);the China Scholarship Council(CSC),Carl Tryggers Stiftelse(No.CTS 19:379);Swedish Government strategic faculty grant in material science(SFO,MATLIU)in Advanced Functional Materials(AFM)(VR No.5.1-2015-5959);the Centre in Nano Science and technology at LiTH(CeNano),and LiU Cancer network at Linköping University.

摘　　要：Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria,thereby pH fluctuation is one of the most significant indicators for tracking mitophagy.Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features,including mitochondrial-specificity,excellent biocompatibility,wide pH-sensitive range of 8.0–4.0,and especially quantitative ability.However,available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule.To fully address this issue,we herein integrate two fluorogenic pH sensitive units,a mitochondria-specific block,cellpenetrating facilitator,and biocompatible segments into an elegant silica nano scaffold,which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy.Most significantly,at a single wavelength excitation,the integrated pHsensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH,thus enabling a ratiometric analysis of pH variations during the whole mitophagy.This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.

关 键 词：mitochondrial pH MITOPHAGY ratiometric fluorescence NANOPROBE cell imaging 

分 类 号：TB3[一般工业技术—材料科学与工程]