果胶酶生产及工业应用进展  

Progress on Production and Industrial Application of Pectinase

作　　者：李力群 孙志康 郝捷 季嫱 李选文 吴晗 吴娜 郑超 杨婧 LI Liqun;SUN Zhikang;HAO Jie;JI Qiang;LI Xuanwen;WU Han;WU Na;ZHENG Chao;YANG Jing(Inner Mongolia Kunming Cigarette Limited Liability Company,Huhhot 010020,China)

机构地区：[1]内蒙古昆明卷烟有限责任公司,呼和浩特010020

出　　处：《生物技术进展》2022年第4期549-558,共10页Current Biotechnology

摘　　要：果胶酶是水解酶家族成员,也是生物技术领域的重要酶,其在全球工业酶市场中所占份额约为25%。果胶酶在工业生产中应用广泛,如植物纤维的脱胶、茶和咖啡的发酵、废水处理、纸浆漂白和动物饲料生产等。在果胶酶的天然来源中,由于微生物具有独特的理化性质,最常被用以生产果胶酶。然而,与许多其他工业酶一样,果胶酶也存在野生菌株产量低、工业生产率低等制约因素,因此,目前果胶酶的研究重点主要集中在如何提高工业规模的生产水平。主要介绍了果胶酶的天然来源,以及在这些来源的基础上通过基因工程改造以获得果胶酶高效表达的最新策略,并概括总结了果胶酶发酵工艺和工业应用,以期为生产具有高活性的果胶酶,提高工业生产的效益奠定理论基础。Pectinase,a member of the hydrolase family,which is the important enzymes in the field of biotechnology,accounting for 25%of the global industrial enzyme market.Pectinase is widely used in industrial production,such as degumming of plant fibers,fermentation of tea and coffee,wastewater treatment,pulp bleaching and animal feed production.Among the natural sources of pectinase,microorganisms are most often used to produce pectinase due to their unique physical and chemical properties.However,like many other industrial enzymes,pectinase also has constraints such as low yield and low industrial productivity of wild strains.Therefore,the current research of pectinase focused on how to improve the industrial scale production level.This review mainly introduced the natural sources of pectinase and the latest strategies of obtaining high expression of pectinase through genetic engineering on the basis of these sources,and summarized the fermentation process and industrial application of pectinase,in order to lay a theoretical foundation for producing pectinase with high activity and improving the benefits of industrial production.

关 键 词：果胶酶 菌种改良 微生物发酵 工业应用 

分 类 号：TS201.25[轻工技术与工程—食品科学] Q552[轻工技术与工程—食品科学与工程]