多种渔用麻醉剂检测快速前处理方法的研究与建立  

Research and establishment of rapid pretreatment method for the detection of various fishery anesthetics

作　　者：陆亦宽 徐迪 刘文悦 郭桂萍 卢瑛[1,2,3] 谢晶 LU Yikuan;XU Di;LIU Wenyue;GUO Guiping;LU Ying;XIE Jing(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306,China;Laboratory of Quality&Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China;Nantong Customs District P.R.China,Nantong 226000,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [4]中华人民共和国南通海关,江苏南通226000

出　　处：《食品与发酵工业》2022年第14期244-250,共7页Food and Fermentation Industries

基　　金：2019年上海市科技兴农重点攻关项目(2019-02-08-00-10-F01143);南京海关项目(2021KJ23)。

摘　　要：该研究通过对渔用麻醉剂三卡因的前处理方法的优化和验证,建立了一种适用于三卡因、2-苯氧乙醇、丙泊酚、丁香酚、乙酸丁香酚酯、苯佐卡因6种渔用麻醉剂检测的快速前处理方法。首先通过优化检测波长和流动相建立了多种渔用麻醉剂的HPLC检测方法,检测波长为220 nm和280 nm同时检测,流动相为乙腈-水(体积比7∶3);然后,通过减少提取试剂用量、简化样品提取和净化浓缩流程,将前处理时间缩短至20 min内;再通过单因素优化三卡因前处理条件,发现在提取剂乙腈添加量1.5 mL、涡旋混合时间2.5 min、净化剂用量150 mg的条件下,三卡因的平均回收率为(89.46±2.42)%。采用6种渔用麻醉剂、3种水产基质(乌鳢、南美白对虾、海鲈鱼)进行提取效率评价,回收率为82.00%~103.87%。三卡因加标样品和麻醉大菱鲆模拟运输制备的实际样品检测发现,该研究所建立的前处理方法适用于HPLC和试纸条检测,具有操作简单、耗时短,适用范围广等特性,为我国水产品中兽药残留等化学危害物的快速现场检测配套样品前处理方法的建立提供了新途径。Through the optimization and verification of the pretreatment method of tricaine,a rapid pretreatment method suitable for the detection of 6 kinds of fishery anesthetics,including tricaine,2-phenoxyethanol,propofol,eugenol,eugenol acetate and benzocaine was established.Firstly,a HPLC method for the determination of various fishery anesthetics was established by optimizing the detection wavelength and mobile phase.The detection wavelength was 220 nm and 280 nm,and the mobile phase was acetonitrile-water(volumeratio 7∶3).Secondly,by reducing the amount of extraction reagent and simplifying the process of sample extraction,purification and concentration,the pretreatment time was shortened to less than 20 min.Then the pretreatment conditions of tetracaine were optimized by single factor.It was found that the average recovery of tetracaine was(89.46±2.42)%under the conditions of 1.5 mL of acetonitrile,2.5 min of vortex mixing time and 150 mg of purifying agent.Six kinds of fishery anesthetics and three kinds of aquatic substrates(Ophiocephalus argus,Penaeus vannamei and sea bass)were used to evaluate the extraction efficiency.The recovery was 82.00%-103.87%.Besides,it was found that the pretreatment method established in this study was suitable for HPLC and test strip detection.It had the characteristics of simple operation,short time-consuming and wide application range.It provides a new way for the establishment of supporting sample pretreatment methods for the rapid on-site detection of veterinary drug residues and other chemical hazards in aquatic products in China.

关 键 词：渔用麻醉剂 检测 快速前处理 高效液相色谱 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]