响应面法优化酶解制备皖鱼皮胶原蛋白活性肽工艺及产物表征  

Optimization of enzymolysis preparation process of collagen active peptide from Wan fish skin by response surface methodology and its product characterization

作　　者：王振强[1] 田振华 王皓 WANG Zhenqiang;TIAN Zhenhua;WANG Hao(Yellow River Conservancy Technical Institute,Kaifeng 475003;Department of Food Engineering and Biotechnology,Tianjin University of Science&Technology,Tianjin 300457)

机构地区：[1]黄河水利职业技术学院,开封475003 [2]天津科技大学食品科学与工程学院,天津300457

出　　处：《中国食品添加剂》2022年第8期80-86,共7页China Food Additives

基　　金：河南省高等学校青年骨干教师培养计划(2017GGJS224)。

摘　　要：以皖鱼皮为原料,水解度(Degree of Hydrolysis,DH)为评价指标,响应面法优化酶解制备胶原蛋白活性肽工艺,为皖鱼皮胶原蛋白活性肽开发利用提供理论参考。结果表明,最佳制备工艺条件:水解温度50℃、pH 10、加酶量2000U、料液比1∶3(g/mL),在此条件下进行验证试验,水解度为28.16%。水解液经超滤和喷雾干燥后,得到的水解产物为有苦涩味的白色粉末,主要是小分子寡肽,分子量主要分布在200~1000Da,占比达到82.39%。Using Wan fish skin as the raw material,degree of hydrolysis(DH)was used as an evaluation index,enzymolysis preparation process of collagen active peptide from Wan fish skin was optimized by response surface methodology.The study provided a theoretical reference for comprehensive utilization of collagen active peptide from Wan fish skin.The results showed that the best preparation process was hydrolysis temperature 50℃,pH 10,enzyme amount 2000U,material-to-liquid ratio 1∶3(g/mL).The validation test showed that DH was 28.16%under these conditions.After ultrafiltration and spray drying,the hydrolysates were white powder with bitter taste,mainly small molecular oligopeptides and its molecular weight was between 200~1000Da,percentage was 82.39%.

关 键 词：皖鱼皮 胶原蛋白活性肽 酶解 响应面 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]