Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish  

作　　者：Hong Zhang Wan-Wan Zhang Cheng-Yu Mo Meng-Dan Dong Kun-Tong Jia Wei Liu Mei-Sheng Yi 

机构地区：[1]School of Marine Sciences,Sun Yat-Sen University,Guangzhou,Guangdong 510275,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai,Guangdong 519000,China [3]Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering,Guangzhou,Guangdong 510000,China

出　　处：《Zoological Research》2022年第4期537-551,共15页动物学研究（英文）

基　　金：supported by the National Key R&D Program of China(2018YFD0901205);National Natural Science Foundation of China(31771587,31970535);Guangdong Basic and Applied Basic Research Foundation(2020A1515010358)。

摘　　要：In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long generations.Nevertheless,functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture fish.In this study,we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers(Bostrychus sinensis),which are prone to ovotesticular or sterile testicular development,and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional(3D)culture system.Artificial insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal larvae.Furthermore,melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway,and thus increased the efficiency in functional sperm production.The 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.

关 键 词：In vitro spermatogenesis 3D culture model SPERMATOGONIA Four-eyed sleeper MELATONIN Genetic breeding 

分 类 号：S917.4[农业科学—水产科学]