UV-B辐照对特早熟柑橘采后果皮色素的影响  

Effect of UV-B Irradiation on Peel Pigment of Postharvest Early-maturing Citrus

作　　者：杨慧 郭世泽 伍峥 孙志伟 唐天睿 杨学虎 YANG Hui;GUO Shize;WU Zheng;SUN Zhiwei;TANG Tianrui;YANG Xuehu(College of Horticulture and Landscape,Yunnan Agricultural University,Kunming,Yunnan 650100;College of Tropical Crop Science,Yunnan Agricultural University,Pu’er,Yunnan 665000,China)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650100 [2]云南农业大学热带作物学院,云南普洱665000

出　　处：《贵州农业科学》2022年第8期64-69,共6页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目“结果枝着生叶片对特早熟柑桔糖分积累的作用机理”(31660556);云南省教育厅科学研究基金项目“UV-B辐照增强对特早熟柑橘黄酮的影响”(2022Y257)。

摘　　要：【目的】探明特早熟柑橘采后UV-B辐照处理对果皮色素的影响,为云南特早熟柑橘采后外观品质的改善提供依据。【方法】以云南特早熟柑橘兴津为试验材料,通过使用辐照强度为100μW/cm^(2)的中波紫外线(UV-B)对其进行采后处理,处理时间为0 h(CK)、24 h和48 h,并在贮藏期定期(0 d、10 d、20 d、30 d)取样测定果皮中的叶绿素、类胡萝卜素、色素代谢相关基因相对表达量等指标。【结果】UV-B辐照剂量不同对特早熟柑橘果皮色泽的影响不同。UV-B辐照显著提高特早熟柑橘果皮的明亮度和黄色度,降低叶绿素含量,提高类胡萝卜素含量;提高PaO、RCCR和PSY基因的相对表达量,促进特早熟柑橘贮藏期脱绿(叶绿素降解),辐照48 h有利于果皮贮藏期类胡萝卜素的累积,对提高特早熟柑橘在贮藏期的色泽品质具有重要作用。【结论】特早熟柑橘采后使用辐照强度为100μW/cm^(2)的UV-B处理,可有效改善特早熟柑橘果实的表皮色泽,以UV-B辐照48 h贮藏20 d效果最佳。【Objective】The effect of UV-B irradiation treatment on peel pigment of postharvest early-maturing citrus is explored,which provides basis for postharvest treatment method to improve the appearance and quality of early-maturing citrus in Yunnan Province.【Method】The Yunnan early-maturing citrus‘Xingjin’was used as the test material,and the postharvest treatment was carried out by using ultraviolet B(UV-B)with an irradiation intensity of 100μW/cm^(2),and the treatment time was set to 0 h(CK),24 h and 48 h.During the storage period(0 d,10 d,20 d and 30 d),samples were taken regularly to determine the relative expression of chlorophyll,carotenoids,and gene involved in pigment metabolism in the peel.【Result】Different irradiation intensity of UV-B had different impact on the peel color of early-maturing citrus.UV-B irradiation significantly improved the brightness and yellowness of early-maturing citrus peels,decreased the chlorophyll content and increased the carotenoid content.Irradiation can increase the relative expression of PaO,RCCR and PSY genes,and promote degreening(chlorophyll degradation)during storage of early-maturing citrus.Under UV-B irradiation for 48 h,it was beneficial to the accumulation of carotenoids in peel during storage,and was important to improve the color and quality of early-maturing citrus during storage.【Conclusion】The suggested irradiation treatment to postharvest early-maturing citrus is 100μW/cm^(2) UV-B,which can effectively improve the peel color of early-maturing citrus.UV-B irradiation for 48 h is the best for 20 d of storage.

关 键 词：特早熟柑橘 中波紫外线 贮藏期 果皮色素 

分 类 号：S666.2[农业科学—果树学]