β2-Microglobulin exacerbates neuroinflammation,brain damage,and cognitive impairment after stroke in rats  

作　　者：Feng Chen Jing Liu Fa-Qiang Li Shuai-Shuai Wang Yan-Yan Zhang Yun-Yun Lu Fang-Fang Hu Rui-Qin Yao 

机构地区：[1]Department of Neurology,Xuzhou Cancer Hospital,Xuzhou,Jiangsu Province,China [2]Department of Neurobiology,Xuzhou Key Laboratory of Neurobiology,Xuzhou Medical University,Xuzhou,Jiangsu Province,China [3]Department of Neurology,Xuzhou No.1 People’s Hospital,the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University,Xuzhou,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2023年第3期603-608,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81771337(to RQY).

摘　　要：β2-Microglobulin(β2M),a component of the major histocompatibility complex class I molecule,is associated with aging-related cognitive impairment and Alzheimer’s disease.Although upregulation ofβ2M is considered to be highly related to ischemic stroke,the specific role and underlying mechanistic action ofβ2M are poorly understood.In this study,we established a rat model of focal cerebral ischemia by occlusion of the middle cerebral artery.We found thatβ2M levels in the cerebral spinal fluid,serum,and brain tissue were significantly increased in the acute period but gradually decreased during the recovery period.RNA interference was used to inhibitβ2M expression in the acute period of cerebral stroke.Tissue staining with 2,3,5-triphenyltetrazolium chloride and evaluation of cognitive function using the Morris water maze test demonstrated that decreasedβ2M expression in the ischemic penumbra reduced infarct volume and alleviated cognitive deficits,respectively.Notably,glial cell,caspase-1(p20),and Nod-like receptor pyrin domain containing 3(NLRP3)inflammasome activation as well as production of the inflammatory cytokines interleukin-1β,interleukin-6,and tumor necrosis factor-αwere also effectively inhibited byβ2M silencing.These findings suggest thatβ2M participates in brain injury and cognitive impairment in a rat model of ischemic stroke through activation of neuroinflammation associated with the NLRP3 inflammasome.

关 键 词：cognitive impairment cognitive improvement glial activation infarct volume ISCHEMIA middle cerebral artery occlusion NEUROINFLAMMATION NLRP3 inflammasome STROKE β2 microglobulin 

分 类 号：R749[医药卫生—神经病学与精神病学]