Neuroprotective effects of neural stem cells pretreated with neuregulin1β on PC12 cells exposed to oxygen-glucose deprivation/reoxygenation  

作　　者：Qiu-Yue Zhai Yuan-Hua Ye Yu-Qian Ren Zhen-Hua Song Ke-Li Ge Bao-He Cheng Yun-Liang Guo 

机构地区：[1]Institute of Cerebrovascular Diseases,Medical Research Center,The Affiliated Hospital of Qingdao University,Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province,Qingdao,Shandong Province,China [2]Department of Obstetrics,The Affiliated Hospital of Qingdao University,Qingdao,Shandong Province,China [3]Research Center of Stem Cells,Shandong Haoyun International Hospital of Stem Cells,Jinan,Shandong Province,China

出　　处：《Neural Regeneration Research》2023年第3期618-625,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81973501;the Natural Science Foundation of Shandong Province,No.ZR2019MH009(both to YLG).

摘　　要：Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations.In addition,the survival,differentiation,and proliferation rates of transplanted exogenous NSCs are low,which limit their clinical application.Our previous study showed that neuregulin1β(NRG1β)alleviated cerebral I/R injury in rats.In this study,we aimed to induce human umbilical cord mesenchymal stem cells into NSCs and investigate the improvement effect and mechanism of NSCs pretreated with 10 nM NRG1βon PC12 cells injured by oxygen-glucose deprivation/reoxygenation(OGD/R).Our results found that 5 and 10 nM NRG1βpromoted the generation and proliferation of NSCs.Co-culture of NSCs and PC12 cells under condition of OGD/R showed that pretreatment of NSCs with NRG1βimproved the level of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,nicotinamide adenine dinucleotide phosphate,and nuclear factor erythroid 2-related factor 2(Nrf2)and mitochondrial damage in injured PC12 cells;these indexes are related to ferroptosis.Research has reported that p53 and solute carrier family 7 member 11(SLC7A11)play vital roles in ferroptosis caused by cerebral I/R injury.Our data show that the expression of p53 was increased and the level of glutathione peroxidase 4(GPX4)was decreased after RNA interference-mediated knockdown of SLC7A11 in PC12 cells,but this change was alleviated after co-culturing NSCs with damaged PC12 cells.These findings suggest that NSCs pretreated with NRG1βexhibited neuroprotective effects on PC12 cells subjected to OGD/R through influencing the level of ferroptosis regulated by p53/SLC7A11/GPX4 pathway.

关 键 词：ferroptosis P53 SLC7A11 GPX4 human umbilical cord-mesenchymal stem cells neural stem cells neuregulin1β NEUROPROTECTION oxygen-glucose deprivation/reoxygenation PC12 cell 

分 类 号：R741[医药卫生—神经病学与精神病学]