Trilineage Sequencing Reveals Complex TCRβTranscriptomes in Neutrophils and Monocytes Alongside T Cells  

作　　者：Tina Fuchs Kerstin Puellmann Chunlin Wang Jian Han Alexander W.Beham Michael Neumaier Wolfgang E.Kaminski 

机构地区：[1]Institute for Clinical Chemistry,University of Heidelberg Medical Faculty Mannheim,D-68167 Mannheim,Germany [2]Aesculabor Hamburg,D-22769 Hamburg,Germany [3]iRepertoire Inc.,Huntsville,AL 35806,USA [4]HudsonAlpha Institute for Biotechnology,Huntsville,AL 35806,USA [5]Department of Surgery,Hospital of Siegen,D-57076 Siegen,Germany [6]Ingenium digital diagnostics,D-87662 Kaltental,Germany

出　　处：《Genomics, Proteomics & Bioinformatics》2021年第6期926-936,共11页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by a grant from the Stiftung für Pathobiochemie und Molekulare Diagnostik of the Deutsche Vereinte Gesellschaft für Klinische Chemie und Labormedizin(DGKL),Germany(Grant No.FUCHS 5-2015)。

摘　　要：Recent findings indicate the presence of T cell receptor(TCR)-based combinatorial immune receptors beyond T cells in neutrophils and monocytes/macrophages.In this study,using a semiquantitative trilineage immune repertoire sequencing approach as well as under rigorous bioinformatic conditions,we identify highly complex TCRβtranscriptomes in human circulating monocytes and neutrophils that separately encode repertoire diversities one and two orders of magnitude smaller than that of T cells.Intraindividual transcriptomic analyses reveal that neutrophils,monocytes,and T cells express distinct TCRβrepertoires with less than 0.1%overall trilineage repertoire sharing.Interindividual comparison shows that in all three leukocyte lineages,the vast majority of the expressed TCRβvariants are private.We also find that differentiation of monocytes into macrophages induces dramatic individual-specific repertoire shifts,revealing a surprising degree of immune repertoire plasticity in the monocyte lineage.These results uncover the remarkable complexity of the two phagocyte-based flexible immune systems which until now has been hidden in the shadow of T cells.

关 键 词：MONOCYTE NEUTROPHIL T cell TCRβtranscriptome Next-generation sequencing 

分 类 号：R392[医药卫生—免疫学]