基于酸性离子液体的原位泡腾辅助微萃取法测定饮料与糖果中的亮蓝和赤藓红  

Determination of Brilliant Blue FCF and Erythrosine B in Beverage and Candy Samples Using In-situ Effervescence Assisted Microextraction Method Based on Acidic Ionic Liquid

作　　者：王庆 邱彬[2] 何艳 王玲娟 张辉 杨二玲 张晓元 WANG Qing;QIU Bin;HE Yan;WANG Lin-juan;ZHANG Hui;YANG Er-ling;ZHANG Xiao-yuan(The College of Pharmacy,Xiangnan University,Chenzhou 423000,China;Chenzhou City Center for Disease Control and Prevention,Chenzhou 423000,China;Research Institute of Shaoguan Huagong High-Tech Industry,Shaoguan 512000,China;Industrial Technology Research Institute,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]湘南学院药学院,湖南郴州423000 [2]郴州市疾病预防控制中心,湖南郴州423000 [3]韶关市华工高新技术产业研究院,广东韶关512000 [4]华南理工大学工业技术研究总院,广东广州510640

出　　处：《分析测试学报》2022年第8期1146-1152,共7页Journal of Instrumental Analysis

基　　金：Excellent Youth Fund Project of Hunan Provincial Education Department(20B542);Innovation and EntrepreneurshipTraining Program for College Students in Hunan Province(XJT[2021]No.197-serial number 3669);Horizontal Scientific Research Project(2021E82);Guangdong Science and Technology Plan Project(200729176271266);Hunan Provincial Natural Science (2021JJ80074);Hunan Province Applied Characteristic Discipline Construction Funding Project.

摘　　要：利用酸性离子液体1-丁基-3-甲基咪唑硫酸氢盐([C_(4)MIM][HSO_(4)])的阴离子与碳酸盐反应产生二氧化碳,通过气泡促进萃取过程。在萃取的同时,[C_(4)MIM][HSO_(4)]的阳离子和六氟磷酸铵(NH_(4)PF_(6))原位反应形成疏水性的离子液体1-丁基-3-甲基咪唑六氟磷酸盐([C_(4)MIM][PF_(6)]),从而与水相分离,通过离心收集萃取相,建立了一种基于酸性离子液体的原位泡腾辅助微萃取饮料与糖果样品中亮蓝和赤藓红的方法。考察了酸性离子液体、碳酸氢钠和盐用量对2种色素萃取效率的影响,得到最佳条件为酸性离子液体用量400 mg,碳酸氢钠用量0.4 g,盐用量1.5 g。采用最优条件,目标物亮蓝与赤藓红的质量浓度在0.005~5μg/mL范围内呈良好的线性关系,相关系数不小于0.9933,检出限(LOD)和定量下限(LOQ)分别为0.002~0.005μg/mL和0.007~0.017μg/mL,日内和日间相对标准偏差(RSD)为2.2%~8.7%。在0.05、0.5μg/mL 2个加标水平下,回收率为92.2%~107%。所建方法成功用于饮料和糖果样品中2种目标色素的检测。该方法环保、简单且准确,可用于饮料和糖果样品中亮蓝与赤藓红的测定。The anion of acidic ionic liquid 1-butyl-3-methylimidazole hydrogen sulfate([C_(4)MIM][HSO_(4)])reacted with carbonate to produce carbon dioxide.The extraction process was promoted by carbon dioxide bubbles.Mean⁃while,the cation of[C_(4)MIM][HSO_(4)]reacted in situ with ammonium hexafluorophosphate(NH_(4)PF_(6))to form hydropho⁃bic ionic liquid 1-butyl-3-methylimidazole hexafluorophosphate[C_(4)MIM][PF_(6)].[C_(4)MIM][PF_(6)]was separated from water due to their immiscibility with the water.The extraction phase was collected by centrifugation.An in-situ effer⁃vescent assisted microextraction method based on acidic ionic liquid was established for the analysis of brilliant blue FCF and erythrosine B in beverage and candy samples.Effects of the amount of acidic ionic liquid,sodium bicarbon⁃ate and salt on the extraction efficiency of the two pigments were investigated.The optimum conditions were as follows:the amount of acidic ionic liquid was 400 mg,the amount of sodium bicarbonate was 0.4 g and the amount of salt was 1.5 g.Under the optimal conditions,there were good linear relationships for brilliant blue FCF and erythro⁃sine B in the range of 0.005-5μg/mL,with their correlation coefficients not less than 0.9933.The limits of detec⁃tion(LODs)and limits of quantitation(LOQs)were in the range of 0.002-0.005μg/mL and 0.007-0.017μg/mL,respectively.Additionally,the recoveries for two synthetic pigments at two spiked levels of 0.05 and 0.5μg/mL ranged from 92.2%to 107%,with the intra-day and inter-day relative standard deviations(RSDs)both 2.2%-8.7%.The proposed method was successfully applied to the detection of two target pigments in beverage and candy samples.The results showed that the method was environmentally friendly,simple and accurate.It could be used for the deter⁃mination of brilliant blue FCF and erythrosine B in beverage and candy samples.

关 键 词：离子液体 泡腾辅助微萃取 合成色素 饮料 糖果 

分 类 号：O657.7[理学—分析化学] TS201.6[理学—化学]