海参蒸煮液干粉对四氯化碳致小鼠急性肝损伤的预防作用  

Preventive Effect of Dry Powder of Sea Cucumber Cooking Liquid on Acute Liver Injury Induced by CCl4 in Mice

作　　者：蔡维震 张天骐 王庆慧 丁宁 胡炜 王静凤[1] Cai Weizhen;Zhang Tianqi;Wang Qinghui;Ding Ning;Hu Wei;Wang Jingfeng(Ocean University of China,Qingdao 266000,Shandong;Qingdao Malvern College,Qingdao 266000,Shandong;Shandong Haodangjia Ocean Development Co.,Ltd.,Weihai 264200,Shandong)

机构地区：[1]中国海洋大学,山东青岛266000 [2]青岛墨尔文中学,山东青岛266000 [3]山东好当家海洋发展股份有限公司,山东威海264200

出　　处：《中国食品学报》2022年第7期115-122,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC0311203)。

摘　　要：目的:探究海参蒸煮液干粉(SCCL)对小鼠急性肝损伤的预防作用。方法:采用腹腔注射体积分数为5%的CCl_(4)玉米油溶液,建立急性肝损伤小鼠模型,检测SCCL对小鼠血清、肝脏中抗氧化酶、氧化产物的影响;H&E和8-OH dG免疫组化染色观察小鼠肝脏组织病理学变化;qRT-PCR法测定肝组织Nrf2、Keap-1、HO-1、NQO-1 mRNA的表达情况。结果:相较于模型组,SCCL组小鼠的血清、肝脏中抗氧化酶活力显著上升,蛋白质、脂质氧化产物含量显著减少;小鼠肝组织形态明显改善;Nrf2、Keap-1、HO-1、NQO-1 mRNA表达显著增加。结论:SCCL通过增强肝损伤小鼠的抗氧化能力,抑制机体氧化应激,改善了CCl_(4)诱导的急性肝损伤。Objective:To investigate the protective mechanism of dry powder of sea cucumber cooking liquid on acute liver injury mice,which will provide evidences in developing function food for liver protection.Methods:Acute liver injury models established by intraperitoneal injection of CCl_(4) corn oil solution with a volume fraction of 5%in mice.The activities of antioxidant enzyme and the contents of oxidation product were determined in serum and liver.Liver tissue was stained with H&E and immunohistochemistry for observing the of SCCL on mice liver histopathology.Expression of Nrf2,Keap-1,HO-1,NQO-1 mRNA were measured by qRT-PCR.Results:Compared with model group,the groups of SCCL,antioxidant enzyme activities significantly increased and oxidation product contents were declined significantly in serum and liver;the liver histomorphology was improved;expression levels of Nrf2,Keap-1,HO-1,NQO-1 were improved in the SCCL groups.Conclusion:The SCCL enhanced the antioxidant activity,ameliorateorganism damage by oxidative stress,effectively protect the acute liver injury by CCl_(4) in mice.

关 键 词：海参蒸煮液干粉 急性肝损伤 氧化应激 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]