沙门氏菌的检测与分离鉴定技术研究进展  

Research Progress in Detection,Isolation and Identification of Salmonella

作　　者：温珍玉 张红霞[1] 李美霞 张建民[1] 廖明[1] 瞿孝云 WEN Zhenyu;ZHANG Hongxia;LI Meixia;ZHANG Jianmin;LIAO Ming;QU Xiaoyun(South China Agricultural University,Guangzhou Guangdong 510642)

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2022年第4期66-70,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：国家肉鸡产业技术体系(CARS-41-G16);沃尔玛基金会(61626817);沃尔玛基金会项目(52514289)。

摘　　要：沙门氏菌是重要的人畜共患病病原,不仅造成畜禽养殖业的重大经济损失,其感染后的动物产品也会威胁人类健康,如何有效对其监测和防控具有重大的公共卫生意义。本文通过对当前沙门氏菌的传统培养方法、免疫学方法、分子生物学方法、光谱质谱法、生物传感器等检测方法与分离鉴定技术进行概述和综合比较。每种方法都有其优势与不足,将不同方法联合运用是一个可行的思路。展望未来,检测设备的小型化和自动化是一大趋势,如何开发集预处理和检测于一体的筛选和鉴定方法是未来沙门氏菌检测方法的热门话题。Salmonella is an important zoonotic pathogen,which not only causes great economic losses in livestock and poultry breeding industry,but also its infected animal products will threaten human health. How to effectively monitor and prevent Salmonella has great public health significance. By summarizing the current detection methods of Salmonella,this paper summarizes the following methods:traditional culture methods,immunological methods,molecular biology methods,spectral mass spectrometry,biosensors and so on. Each method has its advantages and disadvantages. It is a feasible idea to combine different methods. Looking forward to the future,the miniaturization and automation of detection equipment is a major trend. How to develop screening and identification methods integrating pretreatment and detection is a hot topic of Salmonella detection methods in the future.

关 键 词：沙门氏菌 分离鉴定 快速检测 血清型 生物传感器 

分 类 号：S852.61[农业科学—基础兽医学]