Peptidome analysis of human intrauterine adhesion tissues and the identification of antifibrotic peptide  

作　　者：Xiangdong Hua Yan Zhang Juan Xu Lu Xu Yaqian Shi Dazhen Yang Xiaoyan Gu Sumin Wang Xuemei Jia Feng Xu Jie Chen Xiaoyan Ying 

机构地区：[1]Department of Gynecology,Women's Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210004,China [2]Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210011,China

出　　处：《The Journal of Biomedical Research》2022年第4期280-296,共17页生物医学研究杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(81701399 to X.H.,81801404 to L.X.and 81702561 to J.C.);Research Innovation Program for Graduates of Jiangsu Province to Y.S.(JX10413759);Maternal and Child Health Research Projects in Jiangsu Province,China to X.Y.(F201513).

摘　　要：Intrauterine adhesion(IUA)is a common clinical endometrial disease,which can severely damage the fertility and quality of life in women.This study aims to find the differentially expressed endogenous peptides and their possible roles in IUA.Liquid chromatography-mass spectrometry was used to identify the peptidomic profiling of IUA tissues,and the differentially expressed peptides were screened out.Using real-time quantitative PCR,Western blotting,and immunocytochemistry staining,the function of six endogenous peptides was verified in vitro.It was found that peptide 6(T6)(peptide sequence:TFGGAPGFPLGSPLSSVFPR)could inhibit the expression of TGF-β1-induced cell fibrosis in human endometrial stromal cell line and primary human endometrial stromal cell at a concentration of 50μmol/L.This study provides new targets for further clarifying the formation and prevention of IUA.

关 键 词：Asherman's syndrome FIBROSIS PEPTIDE stromal cells 

分 类 号：R711.74[医药卫生—妇产科学]