Exosomal miR-218 regulates the development of endometritis in dairy cows by targeting TGIF2/TGF-βpathway  

作　　者：CHANG CHEN LIMIN QIAO KAIJUN GUO YINGQIU WANG MENGYI YUAN BOFAN FU XIAOBO GAO HEMIN NI LONGFEI XIAO XIANGGUO WANG 

机构地区：[1]Animal Science and Technology College,Beijing University of Agriculture,Beijing,102206,China [2]Department of Animal Husbandry and Veterinary,Beijing Vocational College of Agriculture,Beijing,102442,China [3]Beijing Changping Animal Disease Prevention and Control Center,Beijing,102200,China [4]China Institute of Veterinary Drugs Control,Beijing,100000,China

出　　处：《BIOCELL》2022年第11期2415-2423,共9页生物细胞（英文）

基　　金：This research was supported by the National Natural Science Foundation of China(No.31802263);Outstanding Young Talent Project of the Beijing Municipal Party Committee Organization Department(No.2018000020124G081).

摘　　要：Endometritis affects the reproductive capacity of dairy cows and leads to serious economic losses in dairy farming.Clarification of the pathogenesis of endometritis is necessary to improve the reproductive efficiency of dairy cows.Exosomes and their miRNAs have been proven to play an important role in inflammatory regulation.Exosomal miR-218 is a differentially expressed miRNA found in endometrial epithelial cells(EECs)under endometrial inflammation.Therefore,we investigated the expression of miR-218 in the uterine tissue of dairy cows,lipopolysaccharide(LPS)treated EECs,exosomal vesicles,and regulation of exosomal miR-218 by targeting TGIF-2 inducible factor homology frame 2(TGIF2)/transforming growth factor-beta(TGF-β).The expression of miR-218 was suppressed in inflammatory uterine tissues and LPS treated EECs.The expression of TGIF2 and TGF-βin inflammatory uterine tissues and LPS treated EECs was significantly higher than those in healthy uterine tissues and EECs(p<0.01).Interestingly,miR-218 derived from donor cells was found to regulate the expression of the target gene TGIF2 in recipient cells through the fusion of exosomes.Concurrently,the expression of its target gene TGIF2 was also suppressed by miR-218 in donor cells resulting in fewer TGIF2 being transported into recipient cells with exosomal fusion.This may be a novel mechanism of miRNAs-mediated regulation and provides a new reference for analyzing the pathogenesis of endometritis in dairy cows.

关 键 词：Dairy cows ENDOMETRITIS EXOSOMES MIR-218 TGIF2/TGF-β 

分 类 号：R73[医药卫生—肿瘤]