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作 者:LIMIN CHEN XIAOWEI LI TIANJUN HE QUANCONG WU TINGTING CHEN JUN HUANG YAOBIN LU
机构地区:[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021,China [2]Integrated Plant Protection Center,Lishui Academy of Agricultural and Forestry Sciences,Lishui,323000,China [3]College of Ecology,Lishui University,Lishui,323000,China
出 处:《BIOCELL》2022年第3期821-828,共8页生物细胞(英文)
基 金:This study was approved by the Institutional Review Board(IRB)Institutional of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences.The approval code is 2020AWE061801;The approval date is June 19,2020.
摘 要:Venom plays several important roles in the life of the fire ant,Solenopsis invicta Buren.Laboratory rearing significantly affected the toxin component of S.invicta.However,the molecular mechanism of the change of venomous components when kept in the laboratory is unclear.In this study,RNA sequencing technique(RNA-Seq)was performed to explore differentially expressed genes in the venom gland of S.invicta at 0,10,and 60 days after laboratory rearing.The RNA-Seq results showed that the expression of a large number of genes changed.The DEGs were involved in multiple pathways,including proteolysis,serine-type endopeptidase,and allergen.Furthermore,RNA-Seq and qRT-PCR data revealed that the expression of some genes related to proteolysis and allergen significantly decreased.Thus,our data generated new data relating to toxin-component and the transcriptome dynamics in the venom gland of S.invicta during laboratory rearing.
关 键 词:Solenopsis invicta Buren RNA-SEQ VENOM Laboratory rearing
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