Knockdown Wiskott-Aldrich syndrome protein family member 3(WASF3)inhibits colorectal cancer metastasis and sensitizes to cisplatin through targeting ZNF471  

作　　者：ZHIYONG ZHANG YAN PAN YAN ZHAO MUDAN REN YARUI LI YUN FENG GUIFANG LU SHUIXIANG HE 

机构地区：[1]Gastroenterology,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an,710032,China [2]Cancer Biology,National Clinical Research Center for Digestive Diseases,and Xijing Hospital of Digestive Diseases,Fourth Military Medical University,Xi’an,710032,China

出　　处：《BIOCELL》2022年第8期1917-1924,共8页生物细胞（英文）

基　　金：Key Research and Development Program of Shaanxi(Program No.2019SF-023);Basic Research Program of Natural Science in Shaanxi Province(Program No.2020JM-381)for their financial support.

摘　　要：:Colorectal cancer(CRC)is a heterogeneous cancer,and many risk factors for colorectal cancer have been established.For CRC metastasis,tumor cell migration,adhesion as well as invasion are important processes.Wiskott-Aldrich syndrome protein family member 3(WASF3)is necessary for metastasis of various types of cancers.However,its role in CRC progression has not been fully elucidated.This study examined the in vitro functional roles of WASF3 in the CRC and explored the underlying molecular mechanisms.We used siRNA-WASF3 to gene silence WASF3 in colon cancer cell(HCT116)in vitro.The effects of WASF3 silencing on HCT116 cell apoptosis,proliferation,migration,as well as invasion were assessed by flow cytometry,CCK-8,and transwell assays.ZNF471 protein expressions were detected by immunofluorescence staining and RT-PCR.Moreover,the effects of ZNF471 were studied on a series of in vitro antitumor-promoting assays using HCT116.WASF3 knockdown expression using small interfering RNA(siRNA)ameliorated CRC cell proliferation,anchorage-independent growth,invasion,and metastasis.Furthermore,we observed that WASF3 contributed to upregulating the metastasis signaling pathway through inhibiting the expression of ZNF471.Our study suggests that targeting WASF3 signaling might be a novel therapeutic strategy for treating CRC.

关 键 词：WASF3 Colorectal cancer ZNF471 Proliferation METASTASIS 

分 类 号：R73[医药卫生—肿瘤]