Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses  

作　　者：Ruona Shi Zhenhuan Feng Xiaofei Zhang 

机构地区：[1]CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Center for Cell Lineage and Development,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Center for Cell Lineage and Atlas,Bioland Laboratory,Guangzhou Regenerative Medicine and Health Guangdong Laboratory,Guangzhou 510530,China

出　　处：《Genomics, Proteomics & Bioinformatics》2021年第5期707-726,共20页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the Guangdong Science and Technology Project,China(Grant Nos.2018B030306047 and 2020B1212060052);the National Natural Science Foundation of China(Grant Nos.31770889 and 31801180);the Guangzhou Science and Technology Project,China(Grant No.201904010469);the Guangzhou Regenerative Medicine and Health Guangdong Laboratory project,China(Grant No.2018GZR110104003)。

摘　　要：The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection is currently a global pandemic.Extensive investigations have been performed to study the clinical and cellular effects of SARS-CoV-2 infection.Mass spectrometry-based proteomics studies have revealed the cellular changes due to the infection and identified a plethora of interactors for all SARS-CoV-2 components,except for the longest non-structural protein 3(NSP3).Here,we expressed the full-length NSP3 proteins of SARS-CoV and SARS-CoV-2 to investigate their unique and shared functions using multi-omics methods.We conducted interactome,phosphoproteome,ubiquitylome,transcriptome,and proteome analyses of NSP3-expressing cells.We found that NSP3 plays essential roles in cellular functions such as RNA metabolism and immune response(e.g.,NF-κB signal transduction).Interestingly,we showed that SARS-CoV-2 NSP3 has both endoplasmic reticulum and mitochondrial localizations.In addition,SARS-CoV-2 NSP3 is more closely related to mitochondrial ribosomal proteins,whereas SARS-CoV NSP3 is related to the cytosolic ribosomal proteins.In summary,our integrative multi-omics study of NSP3 improves the understanding of the functions of NSP3 and offers potential targets for the development of anti-SARS strategies.

关 键 词：NSP3 SARS-CoV-2 SARS-COV Multi-omics MITOCHONDRIA 

分 类 号：R373[医药卫生—病原生物学]