火麻籽粕多酚微波辅助提取工艺及其抗氧化活性  

Microwave-assisted Extraction Process and Antioxidant Activity of Polyphenols from Hemp Seed Meal

作　　者：孙晓波 刘卫萍 王小明 张鹏 郭松[1,2] SUN Xiao-bo;LIU Wei-ping;WANG Xiao-ming;ZHANG Peng;GUO Song(College of Food and Biochemical Engineering,Guangxi Science and Technology Normal University,Laibin 546199,Guangxi,China;Key Laboratory for Zhuang and Yao Pharmaceutical Quality Biology,Guangxi Science and Technology Normal University,Laibin 546199,Guangxi,China)

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546199 [2]广西科技师范学院壮瑶药品质生物学重点实验室,广西来宾546199

出　　处：《食品研究与开发》2022年第16期111-118,共8页Food Research and Development

基　　金：2018-2020年广西本科高校特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西科技师范学院大学生创新创业训练计划项目(X2021246)。

摘　　要：为探究火麻籽粕多酚的提取工艺及评价其抗氧化活性,在单因素试验基础上,通过响应面试验优化火麻籽粕中多酚的微波辅助提取工艺,并从DPPH自由基清除能力、羟基自由基清除能力、超氧阴离子自由基清除能力和铁离子还原能力4个方面来评价其抗氧化活性。结果表明,最佳提取工艺条件为乙醇体积分数58%、微波功率311 W、微波时间3.2 min、微波温度50℃、液料比50∶1(mL/g),在此条件下火麻籽粕多酚实际提取量为5.86 mg/g,与理论提取量相对误差仅为0.34%。试验所选浓度范围内,相较于VC,火麻籽粕多酚对DPPH自由基、羟基自由基、超氧阴离子自由基的清除能力更强,同时还具有较强的还原能力。This paper aims to investigate the extraction process of polyphenols from hemp seed meal and evaluate the antioxidant activity.Based on the single-factor test,the microwave-assisted extraction process of polyphenols from hemp seed meal was optimized by response surface methodology.The antioxidant activity was evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,hydroxyl radical scavenging ability,superoxide anion radical scavenging ability,and ferric reducing ability.The results showed that the optimal extraction conditions were 58%ethanol volume fraction,311 W microwave power,3.2 min microwave time,50℃microwave temperature,and 50∶1(mL/g)of liquid to material ratio,under which the actual extraction amount of polyphenols from hemp seed meal was 5.86 mg/g,with a relative error of 0.34%compared with the theoretical extraction amount.Within the selected concentration range,polyphenols from hemp seed meal had a stronger scavenging ability on DPPH radicals,hydroxyl radicals,and superoxide anion radicals,and had stronger reduction ability compared with VC.

关 键 词：火麻籽粕 多酚 提取 抗氧化活性 响应面法 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]