Visually Intracellular Detection of Telomerase Activity Based on DNA Strand Displacement Reaction and Gold Nanoparticle Labeling  

作　　者：Yajun Zhang Shen Yan Zhaoxin Chen Xin Jiang Shuang Rao Zhuoran Jiang Shanshan Qin Xiang Zhou Yuhao Du 

机构地区：[1]College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers of Ministry of Education,Wuhan University,Wuhan,Hubei 430072,China

出　　处：《Chinese Journal of Chemistry》2022年第6期693-698,共6页中国化学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.22037004)。

摘　　要：Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and monitoring.Herein,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase activity.The AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence quenching.In the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)n.The extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence signals.It is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 h.In addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.

关 键 词：TELOMERASE Nanoparticles DNA strand displacement INTRACELLULAR FRET 

分 类 号：R318[医药卫生—生物医学工程] O657.3[医药卫生—基础医学] TB383.1[理学—分析化学]