Coexpression of TLR9 and VEGF-C is associated with lymphatic metastasis in prostate cancer  

作　　者：Xian-Zi Zeng Zhan-Sen Huang Hong-Peng Fang Jie-Ying Wu Qun-Xiong Huang Chu-Bin Zhuang Jing Zhou Jin-Ming Di 

机构地区：[1]Department of Urology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China [2]Department of Infertility and Sexual Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China [3]Department of Pathology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China

出　　处：《Asian Journal of Andrology》2022年第4期380-385,共6页亚洲男性学杂志（英文版）

基　　金：supported by grants from the Natural Science Foundation of Guangdong Province(No.2018A030313261 and No.2021Al515010129);Guangdong Medical Science and Technology Research Foundation(No.A2018079);the National Natural Science Foundation of China(No.81772752).

摘　　要：Prostate cancer(PCa)is one of the most frequent cancers in men,and its biomolecular targets have been extensively studied.This study aimed to analyze the expression of toll-like receptor 9(TLR9)and vascular endothelial growth factor C(VEGF-C)and the clinical value of the coexpression of TLR9 and VEGF-C in PCa.We retrospectively evaluated 55 patients with clinically localized,intermediate-risk,or high-risk PCa who underwent laparoscopic radical prostatectomy(LRP)and extended pelvic lymph node dissection(ePLND)without neoadjuvant hormonal therapy at a single institution from June 2013 to December 2016.In all 55 patients,the median number of lymph nodes(LNs)resected was 23(range:18-31),and a total of 1269 LNs were removed,of which 78 LNs were positive.Seventeen patients had positive LNs,with a positive rate of 30.9%.In addition,the immunohistochemical results in the above patients revealed that high TLR9 expression was correlated with higher Gleason score(GS)(P=0.049),increased LN metastasis(P=0.004),and more perineural invasion(PNI)(P=0.033).Moreover,VEGF-C expression was associated with GS(P=0.040),pathological stage(pT stage)(P=0.022),LN metastasis(P=0.003),and PNI(P=0.001).Furthermore,a significant positive correlation between TLR9 and VEGF-C was found(P<0.001),and the TLR9/VEGF-C phenotype was associated with LN metastasis(P=0.047).Collectively,we propose that TLR9 stimulation may promote LN metastasis in PCa cells through the upregulation of VEGF-C expression,thereby affecting the prognosis of PCa patients.Therefore,these markers may serve as valuable targets for the treatment of PCa.

关 键 词：biochemical progression-free survival COEXPRESSION lymphatic metastasis prostate cancer toll-like receptor 9 vascular endothelial growth factor C 

分 类 号：R737.25[医药卫生—肿瘤] R943[医药卫生—临床医学]