β-1,2-Mannan-based glycoconjugates as potential antifungal vaccines  

作　　者：Jun Liao Bo Pan Xiaobin Zhuo Guochao Liao Yun Gao Zhenzhen Yao Lianghua Wang Qiuye Wu Weihua Pan Binghua Jiao Qingjie Zhao 

机构地区：[1]Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China [2]School of Pharmacy,Second Military Medical University,Shanghai 200433,China [3]Department of Dermatology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China [4]International Institute for Translational Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China

出　　处：《Chinese Chemical Letters》2022年第9期4345-4349,共5页中国化学快报（英文版）

基　　金：financially supported by the Natural Science Foundation of China (Nos. 21502223, 81773580)

摘　　要：Phosphorylated di-,tri-and tetra-saccharides ofβ-1,2-mannan antigen derived from Candida albicans(C.albicans)cell wall were synthesized and covalently conjugated with keyhole limpet hemocyanin(KLH)and human serum albumin(HSA)via a bifunctional linker under mild conditions.The semi-syntheticβ-1,2-mannoside–KLH conjugates were evaluated for the immunization of BALB/c mice.The ELISA results revealed that all three conjugates could elicit high levels of specific IgG antibodies and the acquired antisera could effectively identify theβ-1,2-mannan epitope.Furthermore,the immunofluorescence and flow cytometry assays also uncovered that the induced antibodies,especially that obtained from immunization withβ-1,2-mannotriose–KLH conjugate(1b),could bind well to fungi cell.Eventually,the structure–immunogenicity relationship analysis ofβ-mannan showed that the length of oligo-β-mannoses had a big impact on their immunogenicity andβ-1,2-mannotriose showed the strongest immunogenicity.The results suggested the great potential ofβ-1,2-mannotriose–KLH conjugate as an antifungal vaccine candidate.

关 键 词：β-1 2-Mannan OLIGOSACCHARIDE ANTIFUNGAL GLYCOCONJUGATE Vaccine ANTIGEN 

分 类 号：TQ464[化学工程—制药化工]