高效液相色谱-示差折光检测法检测无刺蜂蜂蜜中海藻酮糖含量  

Determination of Trehalulose in Stingless Bee Honey by High Performance Liquid Chromatography with Refractive Index Detector

作　　者：郑星 王凯[1] 薛晓锋[1] 汪正威 潘鹏 吴黎明[1] 赵亚周[1] 彭文君[1] ZHENG Xing;WANG Kai;XUE Xiaofeng;WANG Zhengwei;PAN Peng;WU Liming;ZHAO Yazhou;PENG Wenjun(Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Xishuangbanna 650223,China;Xishuangbanna Yunfenggushan Biological Technology Co.Ltd.,Xishuangbanna 666200,China)

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]中国科学院西双版纳热带植物园,云南西双版纳650223 [3]西双版纳云蜂古山生物科技有限公司,云南西双版纳666200

出　　处：《食品科学》2022年第16期217-225,共9页Food Science

基　　金：国家自然科学基金面上项目(32172791);国家蜂产业技术体系建设专项(CARS-44)。

摘　　要：目的:建立一种简单快速测定无刺蜂蜂蜜中海藻酮糖含量的分析方法,并基于此方法对不同无刺蜂种、不同地理来源、不同采集时间等条件下所获得的无刺蜂蜂蜜中海藻酮糖的含量进行检测,以期为无刺蜂蜂蜜海藻酮糖的开发和综合利用提供依据,同时也为相关食品标准的建立提供参考依据。方法:通过收集2019—2021年来自不同无刺蜂蜂种、不同地区(中国云南、海南和马来西亚)无刺蜂蜂蜜,利用高效液相色谱-示差折光检测法,对所收集的无刺蜂蜂蜜样品中海藻酮糖含量进行测定。结果:样品用纯水溶解,经0.22μm尼龙滤膜过滤后,以纯水为流动相,离子配体交换柱Hi-Plex Pb(300mm×7.7mm,8μm)为分离色谱柱对无刺蜂蜂蜜中的糖类物质进行分离。Hi-Plex Pb色谱柱可成功将海藻酮糖与蜂蜜中其他寡糖基线分离,检测方法回收率高、重复性好(回收率为93.50%~95.71%,相对标准偏差不高于0.61%),该方法可对无刺蜂蜂蜜样品中的海藻酮糖进行准确分析。结果表明,无刺蜂蜂蜜样品中海藻酮糖质量分数为7.0%~30.8%,不同年度收集的无刺蜂蜂蜜样品中海藻酮糖含量差异显著(P<0.05),且不同蜂种间海藻酮糖含量也具有显著差异(P<0.05)。结论:本研究建立了一种简单、快速、准确分析无刺蜂蜂蜜中海藻酮糖的检测方法,此方法可以应用于无刺蜂蜂蜜中海藻酮糖的测定和无刺蜂蜂蜜的质量控制。实际样品分析结果显示,无刺蜂蜂蜜中含有丰富的海藻酮糖,可作为无刺蜂蜂蜜鉴别和评价的主要品质指标。Objective:This study aimed to establish a high performance liquid chromatography coupled with refractive index detector(HPLC-RID)method for the determination of trehalulose in stingless bee honey,and apply it to actual samples from different stingless bee species,geographic origins,and harvest dates.Methods:We collected stingless bee honey samples produced by different bee species in Yunnan and Hainan as well as Malaysia in 2019-2021 for analysis by HPLC-RID.Results:The samples were dissolved in pure water and filtered through a 0.22μm nylon membrane.Baseline chromatographic separation of trehalulose from other oligosaccharides in honey was achieved on a Hi-Plex Pb column(300 mm×7.7 mm,8μm)using pure water as the mobile phase.The method had high recoveries(ranging from 93.50%-95.71%,with relative standard deviation(RSD)not larger than 0.61%)and good repeatability and accuracy.The content of trehalulose in stingless bee honey ranged from 7.0%to 30.8%,and varied significantly across harvest years and bee species(P<0.05).Conclusion:The proposed method is simple,rapid and accurate and can be used for the determination of trehalulose in stingless honey for quality control purposes.Stingless bee honey is rich in trehalulose,which can be a potential quality index for the identification and evaluation of stingless bee honey.

关 键 词：无刺蜂蜂蜜 无刺蜂蜂种 海藻酮糖 高效液相色谱 示差检测器 质量控制 

分 类 号：TS201[轻工技术与工程—食品科学]