对首届全国病理常规技术染色质量测评结果的分析  

Analysis on the evaluation results of staining quality of pathologically routine technique of the whole nation of the first session

作　　者：薛晓伟[1] 王德田[1] 凌庆[1] 吴世培 刘俊男 宋菲 周炜洵[1] XUE Xiao-wei;WANG De-tian;LING Qing(Department of Pathology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China;不详)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院病理科,北京100730

出　　处：《中国医学装备》2022年第8期33-36,共4页China Medical Equipment

基　　金：中国医学科学院临床与转化医学研究基金(2020-I2M-C&T-B-038)“早期胃癌内镜下黏膜剥离术(ESD)标本人工智能图像复原及辅助病理诊断”。

摘　　要：目的:分析首届全国病理常规技术染色室间质量控制测评结果,探讨影响苏木精-伊红(HE)染色的关键因素。方法:参评专家对全国165家医疗机构的子宫肌瘤、胃肠道、乳腺和肺4种器官组织的测评切片和本单位切片的HE染色质量进行评级。对医疗机构等级中三级医院、二级医院和非公医疗机构3种类型、脱水机品牌中进口脱水机和国产脱水机、脱水试剂的使用中非商品化组织制备试剂和商品化组织制备试剂、HE染色试剂使用中非商品化染色试剂和商品化染色试剂进行HE染色合格率的评价。结果:在医疗机构等级中,三级医院HE染色合格率为84.03%,二级医院HE染色合格率为85.71%,非公医疗机构HE染色合格率为42.86%,公立医疗机构HE染色合格率高于非公医疗机构,差异有统计学意义(x^(2)=7.83,P<0.05)。在脱水机品牌中,进口品牌脱水机的HE染色合格率为82.75%;国产脱水机的HE染色合格率为80%,其差异无统计学意义(x^(2)=0.92,P>0.05)。在脱水试剂使用中,商品化组织制备试剂的HE染色合格率为88.89%,非商品化组织制备试剂的HE染色合格率为80%,其差异无统计学意义(x^(2)=1.71,P>0.05)。在HE染色试剂使用中,商品化染色试剂HE染色合格率为88.89%,非商品化染色试剂的HE染色合格率为90%。其差异无统计学意义(x^(2)=0.02,P>0.05)。结论:HE染色合格率与脱水机品牌,脱水试剂及染色试剂使用无关,与操作人员的染色经验具有相关性。Objective:To analyze the evaluation results of inter-laboratory quality control of the staining of pathologically routine technique of the whole nation of the first session,and to explore the key factors that affected hematoxylin-eosin(HE)staining.Methods:The HE staining qualities of pathological section of 4 kinds of organ and tissue included uterine fibroids,gastrointestinal tract,breast and lung between 165 medical institutions and Peking union medical college hospital were graded by specialists.The qualification rates of HE staining,of the grades of medical institutions included third-level hospital,second-level hospital and non-public healthcare institution,the brands of dehydrators included import dehydrators and domestic dehydrators,the dehydration reagents included non-commercialization tissue preparation reagent and commercialization tissue preparation reagent,and HE staining reagents included non-commercialization staining reagent and commercialization staining reagent,were evaluated.Results:In the comparison of the grades of medical institutions,the qualification rates of HE staining of third-level hospital,second-level hospital and non-public healthcare institution were 84.03%,85.71%and 42.86%,respectively,and the qualification rate of HE staining of publically medical institution was significantly higher than that of non-publically medical institution(x^(2)=7.83,P0.05).In the comparison of dehydration reagents,the qualification rates of HE staining of commercialization and non-commercialization tissue preparation reagent were 88.89%and 80.0%,respectively,and the difference of that between them was no significant(x^(2)=1.71,P>0.05).In the comparison of the reagents of HE staining,the qualification rates of HE staining of commercialization and non-commercialization reagents were 88.89%and 90.00%,respectively,and the difference of that between them was no significant(x^(2)=0.02,P>0.05).Conclusion:The qualification rate of HE staining is no relative with the brand of dehydrator and the uses of dehydratio

关 键 词：苏木精-伊红(HE)染色 质量 测评 影响因素 

分 类 号：R361[医药卫生—病理学]