干酪乳杆菌SB27降低N-二甲基亚硝胺对大鼠肠黏膜细胞损伤作用研究  

Study on Lactobacillus casei SB27 reducing the damage of N-nltrosodimethylamine to intestinal mucosal cells in rats

作　　者：王淑梅 张莉丽[2] 张爽[2] 于佳睿 王雪 单艺 WANG Shu-Mei;ZHANG Li-Li;ZHANG Shuang;YU Jia-Rui;WANG Xue;SHAN Yi(College of Food Engineering,Harbin University,Harbin 150086,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]哈尔滨学院食品工程学院,哈尔滨150086 [2]东北农业大学食品学院,哈尔滨150030

出　　处：《食品安全质量检测学报》2022年第15期4773-4778,共6页Journal of Food Safety and Quality

基　　金：黑龙江省博士后科研启动基金项目(LBH-Q21107);黑龙江省教育科学规划重点课题项目(GJB1422327);黑龙江省自然科学基金项目(LH2020C031);哈尔滨学院青年博士科研启动基金项目(HUDF2021113)。

摘　　要：目的 研究干酪乳杆菌SB27 (Lactobacillus casei SB27)降低N-二甲基亚硝胺(N-nltrosodim ethylamine,NDMA)对大鼠肠道黏膜细胞IEC-6毒性作用的能力。方法 通过CCK-8实验、苏木精-伊红(hematoxylin-eosin,HE)染色、TUNEL染色和透射电镜对干酪乳杆菌SB27降低NDMA对大鼠肠道黏膜细胞IEC-6毒性作用能力进行分析。结果 CCK-8实验结果显示,干酪乳杆菌SB27可减小NDMA致大鼠肠道黏膜细胞IEC-6毒性的影响,且该作用与干酪乳杆菌SB27的剂量有关。HE染色、TUNEL染色和透射电镜实验结果显示, NDMA (80μg/mL)诱导了IEC-6细胞的损伤,干酪乳杆菌SB27(10~4~10~6CFU/mL)可保护IEC-6细胞免受NDMA的毒性损伤。结论 干酪乳杆菌SB27可降低NDMA对大鼠肠道黏膜细胞IEC-6的毒性损伤。Objective To investigate the ability of Lactobacillus casei(SB27) to reduce the toxicity of N-nltrosodimethylamine(NDMA) on intestinal mucosal cells IEC-6 in rats. Methods The ability was analyzed by CCK-8 assay, hematoxylin-eosin(HE) staining, TUNEL staining and transmission electron microscopy, which could reduce the toxicity of Lactobacillus casei SB27 on intestinal mucosal cells IEC-6 in rats induced by NDMA. Results The results of CCK-8 assay showed that Lactobacillus casei SB27 could reduce the toxicity of intestinal mucosal cells IEC-6 induced by NDMA, and the effect was related to the dose of Lactobacillus casei SB27. The results of HE staining, TUNEL staining and transmission electron microscopy showed that NDMA(80 μg/mL) induced the damage of IEC-6 cells, and Lactobacillus casei SB27(10~4-10~6 CFU/mL) could protect IEC-6 cells from the toxic damage of NDMA. Conclusion Lactobacillus casei SB27 can reduce the toxicity damage of NDMA to rat’s intestinal mucosal cells IEC-6.

关 键 词：干酪乳杆菌SB27 N-二甲基亚硝胺 IEC-6细胞 细胞损伤 

分 类 号：TS201.3[轻工技术与工程—食品科学]