Exosome-functionalized magnesium-organic framework-based scaffoldswith osteogenic, angiogenic and anti-inflammatory properties foraccelerated bone regeneration  

作　　者：Yue Kang Chang Xu Ling’ao Meng Xufeng Dong Min Qi Daqing Jiang 

机构地区：[1]Department of Breast Surgery,Cancer Hospital of China Medical University,44 Xiaoheyan Road,Dadong District,Shenyang,110042,PR China [2]School of Materials Science and Engineering,Dalian University of Technology,2 Linggong Road,Ganjingzi District,Dalian,116024,China

出　　处：《Bioactive Materials》2022年第12期26-41,共16页生物活性材料（英文）

基　　金：Natural science Foundation of Liaoning Province,China,grant number of 2020-ZLLH-40.

摘　　要：Exosomes derived from human adipose-derived stem cells (hADSCs-Exos) have shown potential as an effectivetherapeutic tool for repairing bone defects. Although metal-organic framework (MOF) scaffolds are promisingstrategies for bone tissue regeneration, their potential use for exosome loading remains unexplored. In this study,motivated by the potential advantages of hADSCs-Exos and Mg-GA MOF, we designed and synthesized anexosome-functionalized cell-free PLGA/Mg-GA MOF (PLGA/Exo-Mg-GA MOF) scaffold, taking using of thebenefits of hADSCs-Exos, Mg2+, and gallic acid (GA) to construct unique nanostructural interfaces to enhanceosteogenic, angiogenic and anti-inflammatory capabilities simultaneously. Our in vitro work demonstrated thebeneficial effects of PLGA/Exo-Mg-GA MOF composite scaffolds on the osteogenic effects in human bonemarrow-derived mesenchymal stem cells (hBMSCs) and angiogenic effects in human umbilical endothelial cells(HUVECs). Slowly released hADSCs-Exos from composite scaffolds were phagocytosed by co-cultured cells,stabilized the bone graft environment, ensured blood supply, promoted osteogenic differentiation, and acceleratedbone reconstruction. Furthermore, our in vivo experiments with rat calvarial defect model showed thatPLGA/Exo-Mg-GA MOF scaffolds promoted new bone formation and satisfactory osseointegration. Overall, weprovide valuable new insights for designing exosome-coated nanocomposite scaffolds with enhanced osteogenesisproperty.

关 键 词：Metal-organic framework Exosomes Magnesium ions Gallic acid OSTEOGENESIS Angiogenesis ANTI-INFLAMMATION Rat calvarial defect model Bone regeneration 

分 类 号：R318.08[医药卫生—生物医学工程]