HS-SPME-GC-MS法分析3种块菌子囊果主要挥发性成分  

Analyses of volatile compounds of three Tuber species ascocarps by HS-SPME-GC-MS

作　　者：李杨 周冬宇 乔鹏 李佳梅 李忠友 陈娟 LI Yang;ZHOU Dongyu;QIAO Peng;LI Jiamei;LI Zhongyou;CHEN Juan(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China;Shandong Institute of Sericulture,Shandong Academy of Agricultural Sciences,Yantai 264002,Shandong,China;Science,Technology,and Intellectual Property Office of Huidong County,Huidong 615299,Sichuan,China)

机构地区：[1]中囯医学科学院北京协和医学院药用植物研究所,北京100193 [2]山东省农业科学院山东省蚕业研究所,山东烟台264002 [3]会东县科学技术和知识产权局,四川会东615299

出　　处：《菌物学报》2022年第8期1324-1338,共15页Mycosystema

基　　金：中国医学科学院医学与健康科技创新工程(2021-I2M-1-032);国家自然科学基金(81973423);山东省自然科学基金(ZR2020MC001)。

摘　　要：本研究采用顶空-固相微萃取结合气相色谱-质谱联用技术(HS-SPME-GC-MS)比较分析了假喜马拉雅块菌Tuber pseudohimalayense、中华夏块菌T.sinoaestivum和印度块菌T.indicum子囊果主要挥发性有机化合物(volatile organic compounds,VOCs)组成与含量。结果表明,在3种块菌子囊果中共鉴定到173种VOCs,其中假喜马拉雅块菌、中华夏块菌和印度块菌子囊果中分别检测到79、63和71种挥发性成分,3种块菌子囊果中共有成分11种,其中1-辛烯-3-醇是含量最高的共有化合物(39.52%、38.98%和8.46%)。利用挥发性成分对3种块菌种类进行判别分析,结果表明2-丁酮、庚醛、苯乙烯、1-辛烯-3-醇和环十四烷能100.0%地将3种块菌样品按照种类正确分类。本研究初步确定了3种块菌子囊果VOCs组成和含量的差异,并探究了用VOCs相对含量区分3种块菌种类的可行性,为我国块菌质量评价,尤其是加工的块菌商品的鉴别提供了重要的理论依据。The volatile organic compounds(VOCs)of three Tuber species ascocarps were analyzed by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS).Total 173 VOCs were detected in T.pseudohimalayense,T.sinoaestivum and T.indicum ascocarps,including 11 common compounds and the relative content of 1-octen-3-ol is the highest.Principal component analysis and discriminant analysis on the VOCs also supported the feasibility of identifying the three Tuber species by relative content of the ascocarps VOCs.It will be a useful tool for quality evaluation and identification of processed truffle products.

关 键 词：块菌 挥发性有机化合物 气相色谱质谱联用 判别分析 

分 类 号：R284.1[医药卫生—中药学]