16S rDNA实时荧光定量PCR检测大蒜提取物对小鼠肠道菌群的调节作用  

Detection of the effect of Garlic extract in improving intestinal flora in mice using fluorescence quantitative PCR

作　　者：毛旭文[1,2] 李新霞 刘印天[3] 白杰 宋百灵 胡晓霞[4] 肖文俊 李琳琳 MAO Xu-wen;LI Xin-xia;LIU Yin-tian;BAI Jie;SONG Bai-ling;HU Xiao-xia;XIAO Wen-jun;LI Lin-lin(Department of Pharmacy,Xinjiang Medical University,Xinjiang 83001l,China;Key Laboratory of Active Components of Xinjiang Natural Medicine and Drug Release Technology,Xinjiang Medical University,Urumqi,Xinjiang 83001l,China;不详)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]新疆天然药物活性组分与释药技术重点实验室 [3]新疆医科大学第二临床医学院 [4]新疆埃乐欣药业有限公司

出　　处：《中国微生态学杂志》2022年第7期751-758,共8页Chinese Journal of Microecology

基　　金：乌鲁木齐市高新区博士后基金资助;新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713);新疆医科大学第14期大学生创新训练计划项目(CX2019012)。

摘　　要：目的探究蒜氨酸、大蒜辣素、大蒜粉对小鼠肠道菌群的调节作用。方法将SPF级C57BL/6J小鼠分为空白对照组、蒜氨酸组、大蒜辣素组和大蒜粉组,每组10只。空白对照组小鼠灌服蒸馏水30 d,蒜氨酸组、大蒜辣素组、大蒜粉组小鼠灌服不同浓度的受试物溶液30 d,采用16S rDNA实时荧光定量PCR检测实验前后小鼠粪便样本中乳杆菌、双歧杆菌、肠杆菌、肠球菌、产气荚膜梭菌水平变化。结果给药30 d后,与空白对照组相比、实验前后自身对比,蒜氨酸组、大蒜辣素组、大蒜粉组小鼠的肠杆菌、肠球菌水平无明显变化,乳杆菌、双歧杆菌水平显著增加,产气荚膜梭菌水平显著降低。结论蒜氨酸、大蒜辣素、大蒜粉对肠道菌群具有有益的调节作用,是一种良好的天然有益于肠道菌群的食品。Objective To explore the regulatory effect of Garlic extract on intestinal flora of mice. Methods The mice were divided into blank control group, Alliin group, Allicin group and Garlic powder group. Mice in blank control group were treated with distilled water for 30 days;mice in Alliin group, Allicin group and Garlic powder group were treated with different concentrations of tested solution for 30 days. The levels of Lactobacillus, Bifidobacterium, Enterobacteriaceae, Enterococcus and Clostridium Perfringens in feces were measured using 16 S rDNA real-time fluorescence quantitative PCR. Results After 30 days of oral administration of Alliin, Allicin and Garlic powder, the levels of Enterobacteriaceae and Enterococcus had no significant changes;the levels of Lactobacillus and Bifidobacterium increased significantly;the level of Clostridium perfringens decreased significantly. Conclusion Alliin, Allicin and Garlic powder have beneficial regulating effects on intestinal flora, which are good natural food, beneficial to intestinal flora.

关 键 词：蒜氨酸 大蒜辣素 大蒜粉 肠道菌群 16S rDNA荧光定量PCR 

分 类 号：R587.1[医药卫生—内分泌]