脂联素基因多态性与壮族绝经后女性骨质疏松症的相关性  

Association between adiponectin gene polymorphism and osteoporosis in Postmenopausal women of Zhuang nationality

作　　者：周庆辉[1] 王金花[2] 李嘉慧 莫颂秩 Zhou Qinghui;Wang Jinhua;Li Jiahui;Mo Songyi(Department of Somatology and Anatomy,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Histology and Embryology,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of pathogenic Biology and Immunology,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院人体学与解剖学教研室,广西百色533000 [2]右江民族医学院组织学与胚胎学教研室,广西百色533000 [3]右江民族医学院病原微生物学与免疫学教研室,广西百色533000

出　　处：《右江民族医学院学报》2022年第4期493-497,503,共6页Journal of Youjiang Medical University for Nationalities

基　　金：国家自然科学基金资助项目(81560239)。

摘　　要：目的探讨脂联素基因单核苷酸多态性与广西壮族绝经后女性骨质疏松症的相关性。方法用超声骨密度仪对广西373例壮族绝经后女性志愿者测量左侧跟骨的超声振幅衰减,计算出T值,参照骨质疏松症建议诊断标准把志愿者分成骨量正常、骨量减少和骨质疏松3组;采用Multiplex SNaPshot SNP分型方法对脂联素基因rs1063539、rs266729、rs3774261和rs710445多态性位点进行基因分型。分析对比每个位点3种基因型的超声骨密度差异和3种基因型在3组人群中分布频率的差异。结果脂联素基因该4个位点的等位基因分布均符合Hardy-Weinberg平衡;rs1063539 C/G的3种基因型在骨量正常、骨量减少和骨质疏松3组人群中的分布存在明显差异(P<0.05),rs710445 A/G3种基因型之间的超声骨密度存在明显差异(P<0.05)。骨质疏松症危险因素的有序Logistic回归分析显示壮族绝经后女性个体携带rs1063539 CC和CG基因型发生骨质疏松的风险均比GG基因型的高(P<0.05),携带rs710445 AA和AG基因型发生骨质疏松的风险均比GG基因型的低(P<0.05)。结论脂联素基因rs1063539 C/G和rs710445 A/G的基因多态性与广西壮族绝经后女性骨质疏松症相关,rs1063539 CC、CG基因型和rs710445 GG基因型可能是增加骨质疏松风险的遗传因素。Objective To explore the association between adiponectin gene single nucleotide polymorphisms and osteoporosis in postmenopausal women of Zhuang nationality in Guangxi.Methods The ultrasonic bone densitometer was used to measure the broadband ultrasonic attenuation(BUA)of left calcaneus of 373 postmenopausal female volunteers of Zhuang nationality in Guangxi,and the T value was calculated.According to the recommended diagnostic criteria of osteoporosis,the volunteers were divided into three groups:a normal bone mass group,an osteopenia group and an osteoporosis group.The polymorphic loci rs1063539,rs266729,rs3774261 and rs710445 of adiponectin gene were genotyped by Multiplex SNaPshot SNP method.The differences in ultrasound BMD of the three genotypes at each locus and the differences in the distribution frequency of the three genotypes in the three groups were analyzed and compared.Results The distribution of alleles at these four loci of adiponectin gene conformed to Hardy-Weinberg equilibrium.There were significant differences in the distribution of three rs1063539 C/G genotypes among three groups(P<0.05),and there were significant differences in ultrasound BMD among three rs710445 A/G genotypes(P<0.05).The ordered logistic regression analysis of osteoporosis risk factors showed that the risk of osteoporosis in postmenopausal women of Zhuang nationality with rs1063539 CC and CG genotype was higher than that of GG genotype(P<0.05),and the risk of osteoporosis in postmenopausal women of Zhuang nationality with rs710445 AA and AG genotype was lower than that of GG genotype(P<0.05).Conclusion The polymorphism of adiponectin gene rs1063539 C/G and rs710445 A/G is associated with osteoporosis in postmenopausal women of Zhuang nationality in Guangxi.The genotypes of rs1063539 CC,CG and rs710445 GG may be genetic factors that increase the risk of osteoporosis.

关 键 词：脂联素 单核苷酸多态性 骨质疏松症 绝经后女性 壮族 

分 类 号：R394.5[医药卫生—医学遗传学]