广西长寿饮食模式下的蛋白质营养方式对D-半乳糖致衰老小鼠的影响  

Effects of Protein Nutrition Mode under Guangxi Longevity Dietary Pattern on D-Galactose Induced Aging Mice

作　　者：朱雯君 窦君霞[2] 郑文轩 梅丽华 孟宁 于晓涵 韩坤宸 马金克 李锐定 李全阳[1] ZHU Wenjun;DOU Junxia;ZHENG Wenxuan;MEI Lihua;MENG Ning;YU Xiaohan;HAN Kunchen;MA Jinke;LI Ruiding;LI Quanyang(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;Department of Biotechnology Engineering,Taishan Vocational and Technical College,Taian 271000,China)

机构地区：[1]广西大学轻工与食品学院,广西南宁530004 [2]泰山职业技术学院生物技术工程系,山东泰安271000

出　　处：《食品工业科技》2022年第18期371-379,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金(31871802);广西重点研发计划项目(桂科AB18221065)。

摘　　要：本研究以D-半乳糖致衰小鼠为研究对象,在控制能量不变的情况下,设计不同蛋白质含量的饲喂方式,连续饮食干预小鼠8周,通过分析Morris水迷宫试验、脑组织切片及肝组织抗氧化能力,探究广西长寿饮食模式中蛋白质营养所发挥的抗衰老效果。结果表明:P/C(蛋白质/碳水化合物)=1/4组小鼠体重表现最为稳定,相比高(P/C=1/2)、中(P/C=1/3)和低(P/C=1/5)蛋白饮食组,该组小鼠逃避潜伏期显著下降(P<0.05),穿越平台次数和目标象限停留时间以及目标象限路程均显著提高(P<0.05)。与其他处理组相比,低蛋白饮食P/C=1/4组处理能够抑制小鼠肝组织MDA的蓄积,提高总抗氧化活性。观察脑切片发现低蛋白饮食P/C=1/4组小鼠海马区神经元细胞层数多而形态完好,与其它组相比变性、坏死和凋亡的现象得到有效缓解。进一步通过主成分分析创建了综合健康评分,评分排序为:P/C=1/4组>P/C=1/5组>P/C=1/3组>年轻对照组>P/C=1/2组>衰老对照组。综上,P/C=1/4低蛋白饮食组小鼠能够有效抵抗D-半乳糖的致衰伤害,保持较强的学习记忆能力和抗氧化能力,从而展现出较强的抗衰老效果。本研究结果为饮食中蛋白质营养方式与健康长寿的联系提供了理论依据。This research adopted the D-galactose induced senility mice as the research object, under the condition of constant energy control, different protein content of feeding groups was designed. Mice were under dietary intervention for8 weeks, by analyzing the Morris water maze data, brain tissue slice and liver tissue antioxidant capacity to explore the antiaging effect of protein nutrition in Guangxi longevity dietary patterns. The results showed that: Compared with the high(P/C=1/2), medium(P/C=1/3) and low(P/C=1/5) protein diet groups, the escape latency of mice in P/C=1/4(protein/carbohydrate) group significantly decreased(P<0.05). The number of crossing platform, the residence time of target quadrant and the distance of target quadrant significantly increased(P<0.05). Compared with other treatment groups,low protein P/C=1/4 group can inhibit the accumulation of MDA in liver tissue and improve the total antioxidant activity.Observation of brain sections showed that neurons in the hippocampal area of mice in the low protein diet P/C=1/4 group had multiple layers and intact morphology, and the degeneration, necrosis and apoptosis were effectively alleviated compared with other groups. The comprehensive health score was further established by principal component analysis, and the scoring order was as follows: P/C=1/4 group>P/C=1/5 group>P/C=1/3 group>young control group>P/C=1/2group>aging control group. In summary, mice in P/C=1/4 low-protein diet group can effectively resist the senior-induced injury of D-galactose, and maintain strong learning and memory ability and antioxidant ability, thus showing strong antiaging effect. The results of this study would provide a theoretical basis for the relationship between dietary protein nutrition and health as well as longevity.

关 键 词：长寿饮食模式 蛋白质营养方式 D-gal致衰小鼠 氧化应激 学习记忆能力 

分 类 号：TS201.4[轻工技术与工程—食品科学]