基于甲状腺激素及脱碘酶探讨桑葚多酚类物质对壬基酚所致神经行为毒性的干预效应  

Exploring the Intervention Effects of Mulberry Polyphenols on Nonylphenol-Induced Neurobehavioral Toxicity Based on Thyroid Hormone and Deiodinase

作　　者：李中意 柯倩华 刘焕[1,2] 韩文娜 朱思俞 柳春红 LI Zhongyi;KE Qianhua;LIU Huan;HAN Wenna;ZHU Siyu;LIU Chunhong(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Key Laboratory of Food Quality and Safety in Guangdong Province,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]广东省食品质量安全重点实验室,广东广州510642

出　　处：《食品工业科技》2022年第18期390-398,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31770376);广东省自然科学基金面上项目(2021A1515010825);广东省食品质量安全重点实验室(2020B1212060059);广东省现代农业产业技术体系创新团队建设项目(2021KJ130)。

摘　　要：为研究桑葚多酚类物质对壬基酚(Nonylphenol,NP)所致神经行为毒性的干预效果,将70只SPF级SD雄性大鼠随机分为7组:空白对照组(玉米油+生理盐水)、桑葚对照组(玉米油+桑葚粗提液)、NP染毒组(NP+生理盐水)、桑葚干预组(NP+桑葚粗提液)、花青素干预组(NP+花青素)、白藜芦醇干预组(NP+白藜芦醇)、联合干预组(NP+花青素+白藜芦醇),其中桑葚粗提液灌胃量按照120 mg/kg·bw多酚含量计,花青素和白藜芦醇含量与桑葚粗提液中含量相对应。采用旷场实验(Open field test, OFT)和高架十字迷宫实验(Elevated plus maze, EPM)评价桑葚多酚类物质对NP神经行为毒性的干预效果,同时检测甲状腺素(FT3、FT4)、促甲状腺素(TSH)和脱碘酶(DIO1、DIO2、DIO3)水平。结果表明:与NP染毒组对比,四个干预组对NP导致的大鼠体重增长速度下降、食物利用率降低和脏器系数的增加均有一定程度的干预效果(P<0.05或P<0.01),大鼠在OFT中活动的总路程、进入中心区的次数和时间以及EPM中进入开臂次数和时间均显著或极显著大于NP染毒组(P<0.05或P<0.01),并且大鼠血清中FT3、FT4和TSH水平显著提高(P<0.05),肝脏中脱碘酶的含量降低但并无显著性差异。综上,桑葚粗提液、花青素及白藜芦醇可以改善NP所致的一般毒性和神经行为毒性,可能是与其调节甲状腺激素和促甲状腺激素以及脱碘酶水平的作用有关。The purpose of this study was to explore the intervention effects of mulberry polyphenols on neurobehavioral toxicity induced by nonylphenol(NP). 70 SPF male SD rats were randomly divided into seven groups: Blank control group(corn oil+saline), Mulberry control group [corn oil+mulberry crude extract(MCE)], NP exposure group(NP+saline),mulberry group(NP+MCE), anthocyanin group(NP+anthocyanin), resveratrol group(NP+resveratrol), combined group(NP+anthocyanin+resveratrol). The gavage amount of MCE was calculated according to 120 mg/kg·bw polyphenol content,and the anthocyanin and resveratrol content corresponded to the content in MCE. Open field test(OFT) and elevated plus maze(EPM) were used to evaluate the intervention effects of mulberry polyphenols on neurobehavioral toxicity of NP, and the levels of thyroxine(FT3, FT4), thyroid-stimulating hormone(TSH) and deiodinase(DIO1, DIO2, DIO3) were measured. The results showed that compared with NP group, all four intervention groups had varying degrees of intervention effects on the decrease of body weight growth rate, food utilization rate and organ coefficient of rats induced by NP(P<0.05 or P<0.01). The total distance of movement in OFT, the times of entering the central area, the total time of entering the open arm and staying time in EPM were significantly higher in the intervention groups than those in the NP group(P<0.05 or P<0.01), the levels of FT3, FT4 and TSH in serum increased significantly(P<0.05), while the content of deiodinase in liver decreased but had no significant difference. In summary: The MCE, anthocyanin and resveratrol could improve the neurobehavioral toxicity induced by NP, which might be related to the regulation of thyroid hormone,thyrotropin and deiodinase.

关 键 词：壬基酚 桑葚多酚 甲状腺激素 旷场实验 高架十字迷宫 

分 类 号：TS201.4[轻工技术与工程—食品科学]