mRNA序列固有特征与翻译延伸速率的统计相关性  

Statistical correlations between inherent features of mRNA sequences and translation elongation rates

作　　者：王哲 张颖[1] WANG Zhe;ZHANG Ying(College of Science,Inner Mongolian University of Technology,Hohhot 010051,China)

机构地区：[1]内蒙古工业大学理学院,呼和浩特010051

出　　处：《内蒙古工业大学学报（自然科学版）》2022年第4期301-308,共8页Journal of Inner Mongolia University of Technology：Natural Science Edition

基　　金：内蒙古自治区自然科学基金资助项目(2022LHMS03014)。

摘　　要：统计了酿酒酵母基因组中mRNA密码子使用和茎环结构与翻译延伸速率的相关性。结果表明,所有稀有密码子都具有慢速解码的特点,富含GC比富含AU的密码子解码速度更慢。在密码子水平上,纯茎(环)结构对快慢翻译速率的偏好性不明显,而当一个密码子的前2位碱基处于茎结构中,以及第3位碱基处于环结构中时,这类杂合结构显著偏好慢翻译区,上述统计证据支持这样的观点,即mRNA的密码子使用和茎环结构是调节翻译延伸速率的关键因素。The correlations of codon usage and mRNA stem-loop structure with the rates of translation elongation in the Saccharomyces cerevisiae yeast genome are calculated by applying a preference index method based on the binomial distribution defined.The statistical results show that,all rare codons are characterized by slow decoding,and GC-rich codons are decoded more slowly than AU-rich codons.At the codon level,the preference of pure stem(loop)structure of mRNA for translation elongation rates is not significant,whereas when the first 2 bases of a codon are in the stem structure and the 3rd base is in the loop structure,such heterodimeric structures significantly prefer the slow translation region.The above statistical evidence supports the idea that codon usage and stem-loop structure of mRNA are key factors in regulating the rate of translation elongation.

关 键 词：翻译延伸速率 密码子 MRNA二级结构 偏好性指数 

分 类 号：Q-03[生物学]