遗传学实验教学的前沿拓展——等温扩增技术和微流控芯片技术的引入与思考  

Frontier Expansion of Experimental Teaching of Genetics——Introduction and Feedback of Isothermal Amplification Technology and Microfluidic Chip Technology

作　　者：赵雪莹[1,2] 李梦欣 卢大儒[2] 乔守怡[1,2] 皮妍[1,2] ZHAO Xueying;LI Mengxin;LU Daru;QIAO Shouyi;PI Yan(National Demonstration Center for Experimental Biology Education,Fudan University,Shanghai 200433,China;School of Life Sciences,Fudan University,Shanghai 200433,China)

机构地区：[1]复旦大学生物科学国家级实验教学示范中心,上海200433 [2]复旦大学生命科学学院,上海200433

出　　处：《实验室研究与探索》2022年第6期248-251,共4页Research and Exploration In Laboratory

基　　金：复旦大学本科教学研究与改革实践项目(课程思政,FD2021E002/FD2021E003/FD2021E004)。

摘　　要：现代遗传学学科与技术的飞速发展对本科生遗传学实验教学提出了更高的要求。探索如何将新知识、新技术、新案例引入传统的实验教学课程正成为当前教学改革的重要方向。等温扩增技术是经典的聚合酶链式反应技术的拓展和延伸,具有极强的应用性,基于等温扩增的微流控芯片核酸检测技术现已广泛应用于转基因鉴定、病原体鉴定等核酸检测工作中。将基于环介导等温扩增的微流控芯片核酸检测技术快速鉴定转基因材料实验作为遗传学实验教学新案例,能够在拓展学生前沿视野的同时,大大激发学生探索科学的兴趣,培养学生的创新精神和解决实际问题的能力。该实验实施以来,深受学生欢迎,取得了较理想的教学效果,有利于后期进一步实践与推广。The rapid development of modern genetics disciplines and technology has put forward higher requirements for undergraduate genetics experimental teaching.Introducing new knowledge,new technology,and new cases into traditional experimental teaching courses has become an important direction of current teaching reform.Isothermal amplification technology is an expansion and extension of the classic polymerase chain reaction(PCR)technology,and has strong applicability.The microfluidic chip based on isothermal amplification has been widely used in nucleic acid detection such as transgenic detection and pathogen identification.The rapid identification of genetically modified materials experiment based on microfluidic loop-mediated isothermal amplification(LAMP)technology is used as a new case of genetics experimental teaching,which can greatly stimulate students’interest in exploring science and cultivate students’innovative spirit and ability to solve practical problems.Since the implementation of the experiment,it has been well received by students and achieved satisfactory teaching effects,which is conducive to further practice and promotion in the later period.

关 键 词：遗传学实验 环介导等温扩增 微流控芯片 转基因材料鉴定 

分 类 号：Q3-3[生物学—遗传学]