玉米糖肽的体外抗氧化活性  

In vitro Antioxidant Activities of Corn Glycopeptide

作　　者：王晓杰 童玲 刘晓兰 Wang Xiaojie;Tong Ling;Liu Xiaolan(Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology,College of Food and Biological Engineering,Qiqihar University,Qiqihaer 161006)

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,齐齐哈尔161006

出　　处：《中国粮油学报》2022年第8期166-173,共8页Journal of the Chinese Cereals and Oils Association

基　　金：黑龙江省自然科学基金联合引导项目(LH2020C111);黑龙江省省属高等学校基本科研业务费青年创新人才科研项目(135509225)。

摘　　要：利用体外化学法研究糖基化修饰对玉米肽以及体外消化对玉米糖肽抗氧化活性的影响,然后构建H_(2)O_(2)诱导的Caco-2细胞氧化性损伤模型,在细胞水平上对玉米糖肽的抗氧化活性进行研究。结果表明:D-氨基葡萄糖修饰使玉米肽的抗氧化活性显著增加(P<0.05),且在1~2 mg/mL范围内,玉米糖肽的还原力和Fe^(2+)螯合能力显著高于同浓度的GSH和玉米肽(P<0.05)。除Fe^(2+)螯合能力外,体外模拟胃肠消化不能破坏玉米糖肽的自由基清除能力和还原力。当质量浓度为75μg/mL时,玉米糖肽的预处理可以显著提高氧化损伤Caco-2细胞内超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶和γ-谷氨酰半胱氨酸合成酶的活力,从提高细胞抗氧化水平的角度提高了细胞存活率。In the present paper,the effects of glycosylation of corn peptide and in vitro digestion of corn glycopeptide on antioxidant activities were studied,and then,the H_(2)O_(2)-induced oxidative damage model of Caco-2 cells was used to study the antioxidant activity of corn glycopeptide.The result showed that the antioxidant activities of corn peptide modified by D-glucosamine were significantly increased(P<0.05).Moreover,the reducing power and Fe^(2+)-chelating capacity of corn glycopeptide were significantly higher than those of GSH and corn peptide in 1~2 mg/mL(P<0.05).Additionally,except for Fe^(2+)-chelating capacity,it was observed that in vitro digestion did not disrupt corn glycopeptide-related the radicals scavenging abilities and reducing power.The H_(2)O_(2)-induced Caco-2 cell treated with the corn glycopeptide at 75μg/mL showed a significant increase in cell superoxide dismutase,glutathione peroxidase,catalase,andγ-glutamylcysteine synthetase activities,and the viability of Caco-2 cell was improved from the perspective of increasing antioxidant level in cell.

关 键 词：糖基化修饰 体外消化 抗氧化活性 氧化性损伤 细胞存活率 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]