6-苄氨基嘌呤处理对鲜切西兰花硫代葡萄糖苷代谢的影响  

Effects of 6-benzylaminopurine treatments on the glucosinolate metabolism

作　　者：罗淑芬[1] 郭峰[1] 孙莹 王瑶 胡花丽[1] 安容慧 周宏胜[1] 李国锋[1] 李鹏霞[1,2] 无 Luo Shufen;Guo Feng;Sun Ying;Wang Yao;Hu Huali;An Ronghui;Zhou Hongsheng;Li Guofeng;Li Pengxia;无(Institute of Facilities and Equipment in Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Key Laboratory of Cold Chain Logistics Technology for Agro-Products,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农业设施与装备研究所,南京210014 [2]农业农村部农产品冷链物流技术重点实验室,南京210014 [3]江苏省高效园艺作物遗传改良重点试验室,南京210014

出　　处：《农业工程学报》2022年第13期295-304,共10页Transactions of the Chinese Society of Agricultural Engineering

基　　金：江苏省农业科技自主创新资金资助项目(CX(20)1008);国家自然科学基金项目(31901755)。

摘　　要：为探讨6-苄氨基嘌呤(6-Benzylaminopurine,6-BA)处理对鲜切西兰花中硫代葡萄糖苷(简称硫苷)代谢的作用机理,该研究首先鉴定得出西兰花花球中分别以萝卜硫苷和萝卜硫素为主的12种硫苷和6种水解产物,然后以30 mg/L的6-BA溶液对鲜切西兰花进行处理,研究外源6-BA处理对其硫苷含量、黑芥子酶活性、硫苷水解产物及其代谢途径关键基因表达水平等的影响。结果表明,6-BA处理通过延缓西兰花花球叶绿素含量下降从而延迟其黄化进程;同时,显著提高了脂肪族硫苷合成相关基因MYB28、CYP79F1、CYP83A1、ST5b、FMO_(GS-OX1),和吲哚族硫苷合成关键基因MYB51、CYP79B2及CYP83B1的表达水平(P<0.05),其中6-BA处理组FMO_(GS-OX1)的表达量在贮藏第6天时为对照组的1.93倍,MYB51的表达量为对照组1.41~1.91倍,CYP79B2的表达量在4 d后高出对照组41.90%~93.75%;由此,6-BA处理保持了其组织较高的萝卜硫苷、3-甲基亚磺酰丙基硫苷、吲哚甲基硫苷和4-甲氧基吲哚甲基硫苷的含量,总硫苷含量为对照组的2.46~4.79倍;此外,6-BA处理的西兰花花球组织黑芥子酶活性和其基因MYR的表达水平在4~8 d期间分别高出对照组15.32%~90.22%和25.86%~33.73%,并显著提高了其组织萝卜硫素等异硫氰酸酯水解产物的含量(P<0.05),其中萝卜硫素含量高于对照组17.58%~25.39%,但对硫苷水解关键基因ESP的表达量并无显著影响(P>0.05)。因此,6-BA处理可通过提高硫苷合成相关基因和水解关键基因来保持鲜切西兰花花球中硫苷的含量和异硫氰酸酯的水平,为西兰花功能活性成分的保持提供理论和技术支持。This study aims to investigate the underlying mechanism of 6-benzylaminopurine(6-BA) treatment on the glucosinolate(GLS) metabolism of fresh-cut broccoli. Firstly, the individual GLS and the hydrolysis products were identified from the broccoli heads. Twelve types of GLS were detected in the sample, including glucoraphanin(GRA), glucoiberin(GIB),progoitrin(PRO), gluconapin(GNA), glucoerucin(GER), glucoberteroin(GOB), glucotropaeolin(GTR), gluconasturtiin(GNS), glucobrassicin(GBR), 4-hydroxy glucobrassicin(4HGBS), 4-methoxy-glucobrassicin(4MGBS), and neoglucobrassicin(NGBS). Six hydrolysis products of GLS also included the benzonitrile, 4-isothiocyanato-1-butene,4-pentenyl isothiocyanate, butyl-Isothiocyanate, 2-phenylethyl isothiocyanate, and sulforaphane. Among them, the GRA and sulforaphane were the main GLS and hydrolysis product in the broccoli heads. Secondly, the fresh-cut broccoli samples were treated with the 30 mg/L of 6-BA solution for ten minutes, followed by drying at room temperature for two hours, and then stored at(15 ± 1) °C for eight days. The samples treated with distilled water were used as the control. Thirdly, a systematic investigation was made to clarify the effect of 6-BA treatment on the contents of chlorophyll, GLS, and the hydrolysis products,as well as the myrosinase activity of fresh-cut broccoli. The relative expression of genes was also involved in the biosynthesis and degradation of GLS in the broccoli.The results showed that 6-BA treatment significantly delayed the yellowing of the fresh-cut broccoli by inhibiting the degradation of chlorophyll a and b. In addition, it was found that 6-BA treatment up-regulated the relative expression of genes involved in the biosynthesis of aliphatic GLS and indole GLS including MYB28,CYP79F1, CYP83A1, ST5b, and FMO_(GS-OX1), as well as MYB51, CYP79B2 and CYP83B1(P<0.05).Notably, the relative expression of FMO_(GS-OX1), a gene coding for the enzyme responsible for the biosynthesis of GRA, was higher by 1.93 times in 6-BA-treated broccoli than i

关 键 词：贮藏 品质控制 西兰花 6-苄氨基嘌呤 硫苷代谢 基因表达 

分 类 号：S635.3[农业科学—蔬菜学]