地西他滨暴露下2种典型甲藻生长及其抗氧化响应研究  

Studies on Growth and Antioxidant Responses of Two Dinoflagellate Species under Exposure to Decitabine

作　　者：赵建刚[1,2] 唐涛 张建能 郭洁 王朝晖[1,2] Zhao Jiangang;Tang Tao;Zhang Jianneng;Guo Jie;Wang Zhaohui(Department of Ecology,Jinan University,Guangzhou 510632,China;Engineering Research Center of Tropic and Subtropic Aquatic Ecological Engineering,Ministry of Education,Guangzhou 510632,China)

机构地区：[1]暨南大学生态学系,广州510632 [2]热带亚热带水生态工程教育部工程研究中心,广州510632

出　　处：《生态毒理学报》2022年第3期468-476,共9页Asian Journal of Ecotoxicology

基　　金：科技基础资源调查专项(2018FY100200);国家自然科学基金面上项目(42076141)。

摘　　要：地西他滨(decitabine,DAC),是一种DNA甲基转移酶抑制剂,具有一定细胞毒性。为了解DAC对海洋环境及海洋初级生产力的影响,本文研究了DAC对2种典型甲藻塔玛亚历山大藻(Alexandrium tamarense)和血红哈卡藻(Akashiwo sanguinea)的生长及其抗氧化能力的影响。结果表明,在DAC暴露下,2种甲藻细胞生长均受到明显抑制,且DAC浓度越高,抑制效果越明显。DAC对血红哈卡藻和塔玛亚历山大藻生长的96 h半效应浓度(EC_(50))分别为0.0016 mmol·L^(-1)(0.37 mg·L^(-1))和0.116 mmol·L^(-1)(26.47 mg·L^(-1))。藻细胞可溶性蛋白对DAC响应迅速,其含量随时间延长而逐渐升高。超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量均在暴露后24 h达到峰值,然后缓慢下降。DAC对藻细胞谷胱甘肽(GSH)具有促进作用,而且对血红哈卡藻细胞GSH的促进作用更为明显。结果表明,2种甲藻对DAC胁迫响应迅速,相较而言血红哈卡藻敏感性更高,可作为DAC在海洋环境中的指示生物;同时其藻细胞内GSH和MDA含量变化敏感,呈现出明显的剂量-效应和时间-效应关系,可以作为监测DAC环境污染的生物标记指标之一。Decitabine(DAC)is a DNA methyltransferase inhibitor with certain cytotoxicity.Effects of DAC on the growth and antioxidant capacity of Alexandrium tamarense and Akashiwo sanguinea(Dinophyta)were studied by a 96 h growth test in the batch-culture system in order to understand the effects of DAC on marine primary productivity.Results showed that the growth of algal cells was inhibited by DAC in a dose-dependent manner,and the 96-h EC_(50) values of DAC on the growth of Ak.sanguinea and Al.tamarense were 0.0016 mmol·L^(-1)(0.37 mg·L^(-1))and 0.116 mmol·L^(-1)(26.47 mg·L^(-1)),respectively.The soluble protein of the two algal cells responded quickly to DAC,and increased gradually with exposure time.The contents of superoxide dismutase(SOD)and malondialdehyde(MDA)peaked at 24 h exposure and then decreased slowly.DAC enhanced the production of glutathione(GSH)in both algal cells,and more significantly for GSH in Ak.sanguinea.The results suggested that both dinoflagellate species responded quickly to the DAC stress,and Ak.sanguinea showed higher sensitivity which could be used as an indicator of DAC in the marine environment.Furthermore,the contents of GSH and MDA in Ak.sanguinea were sensitive to DAC with a dose-and time-dependent manner,which could be used as potential biomarkers in DAC monitoring.

关 键 词：地西他滨 塔玛亚历山大藻 血红哈卡藻 生长 超氧化物歧化酶(SOD) 谷胱甘肽(GSH) 丙二醛(MDA) 

分 类 号：X171.5[环境科学与工程—环境科学]