正交实验法提取茶叶中咖啡因的工艺研究  

Study on Optimum Route of Extracting Caffeine from Tea by Orthogonal Test

作　　者：王船英 桑蕾 邾枝花[1] 许玉芳[1] WANG Chuan-ying;SANG Lei;ZHU Zhi-hua;XU Yu-fang(Ahhui Medical College,Hefei 230601,China)

机构地区：[1]安徽医学高等专科学校,安徽合肥230601

出　　处：《长春师范大学学报》2022年第8期94-98,共5页Journal of Changchun Normal University

基　　金：安徽省高校科研项目“基于氧化石墨烯量子点的杂化膜制备及其渗透汽化性能的研究”(KJ2021A1263);安徽省线下开放课程“药物分析”(2020kfkc283);安徽省教学示范课“药物分析”(2020SJJXSFJ1386);安徽省示范基层教学组织-化学与药品质量检测教研室(2020SJSFJXZZ231)。

摘　　要：优化茶叶中咖啡因的提取工艺,提高咖啡因产量。设计单因素实验,考察单因素对咖啡因提取率的影响。按单因素的影响大小确定正交实验的考察因素,即以茶叶种类、乙醇体积分数、提取时间、升华时间这4个因素为正交实验考察因素,以咖啡因质量作为评价指标,设计4因素3水平正交实验。结果表明,实验选用红茶品种,95%乙醇作为提取剂,提取120 min,升华10 min,咖啡因质量最高,为76.96 mg。4个因素对咖啡因产率的影响程度由大到小顺序为茶叶种类、提取时间、乙醇体积分数、升华时间。This paper is to optimize the extraction process of caffeine in tea and improve the yield of caffeine.A single factor experiment was designed to investigate the effect on the extraction rate of caffeine.The factors of orthogonal experiment were determined according to the influence of four factors including tea species,ethanol concentration,extraction time and sublimation time.Caffeine yield was taken as the evaluation index,and a four-factor three-level orthogonal experiment was designed.The results showed that the highest yield of 76.96 mg was obtained when black tea was extracted with 95% ethanol for 120 min and sublimated for 10 min.The influence degree of four factors on caffeine yield in descending order was tea species,extraction time,ethanol concentration and sublimation time.

关 键 词：正交实验 茶叶 咖啡因 

分 类 号：TS272.4[农业科学—茶叶生产加工]